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Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens

View ORCID ProfileMichael C. Gilmore, View ORCID ProfileFelipe Cava
doi: https://doi.org/10.1101/2022.07.15.499691
Michael C. Gilmore
Laboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, 90187 Umeå, Sweden
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Felipe Cava
Laboratory for Molecular Infection Medicine Sweden (MIMS), Department of Molecular Biology, Umeå University, 90187 Umeå, Sweden
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  • For correspondence: felipe.cava@umu.se
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Abstract

During growth and division, the bacterial cell wall peptidoglycan (PG) is remodelled, resulting in the liberation of PG muropeptides which are typically reinternalized and recycled. Bacteria belonging to the Rhizobiales and Rhodobacterales orders of the Alphaproteobacteria lack the canonical muropeptide transporter AmpG, despite having other key PG recycling enzymes. Here, we show that an alternative transporter, YejBEF-YepA, takes over this role in the Rhizobiales phytopathogen Agrobacterium tumefaciens. Muropeptide import by YejBEF-YepA governs expression of the β-lactamase AmpC in A. tumefaciens, contributing to β-lactam resistance. However, we show that the absence of YejBEF-YepA causes severe cell wall defects that go far beyond lowered AmpC activity. Thus, contrary to previously established Gram-negative models, PG recycling is vital for cell wall integrity in A. tumefaciens. YepA is widespread in the Rhizobiales and Rhodobacterales, suggesting that YejBEF-YepA-mediated PG recycling could represent an important but overlooked aspect of cell wall biology in these bacteria.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Figure 2 revised. Abstract updated to be more concise

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 03, 2022.
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Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens
Michael C. Gilmore, Felipe Cava
bioRxiv 2022.07.15.499691; doi: https://doi.org/10.1101/2022.07.15.499691
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Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens
Michael C. Gilmore, Felipe Cava
bioRxiv 2022.07.15.499691; doi: https://doi.org/10.1101/2022.07.15.499691

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