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Acinetobacter baumannii defends against oxidative stress through a Mn2+-dependent small RNA-mediated suppression of type VI secretion system

View ORCID ProfileSomok Bhowmik, Avik Pathak, Kuldip Devnath, Tarun Kumar Sharma, Ramandeep Singh, Saurabh Chugh, View ORCID ProfileRanjana Pathania
doi: https://doi.org/10.1101/2022.07.30.502137
Somok Bhowmik
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand-247667, India
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  • ORCID record for Somok Bhowmik
Avik Pathak
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand-247667, India
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Kuldip Devnath
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand-247667, India
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Tarun Kumar Sharma
3Translational Health Science and Technology Institute, Faridabad, Haryana-121001, India
4Department of Medical Biotechnology, Gujarat Biotechnology University, Gujarat-382355, India
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Ramandeep Singh
3Translational Health Science and Technology Institute, Faridabad, Haryana-121001, India
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Saurabh Chugh
3Translational Health Science and Technology Institute, Faridabad, Haryana-121001, India
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Ranjana Pathania
1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand-247667, India
2Centre of Excellence in Disaster Mitigation and Management, Indian Institute of Technology Roorkee, Uttarakhand-247667, India
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  • For correspondence: ranjana.pathania@bt.iitr.ac.in
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SUMMARY

Acinetobacter baumannii employs a plethora of strategies during infection to compete with other pathogens and mitigate host-mediated oxidative stress. A. baumannii utilizes the type VI secretion system (T6SS) to induce contact-dependent killing off the competitor microbes. However, the role of T6SS during host-induced oxidative stress is not explored in A. baumannii. Here, we show that A. baumannii T6+ cells cannot cope with phagocytic cell-mediated oxidative stress due to inadequate uptake of Mn2+, which is crucial for bacterial physiology and reactive oxygen species (ROS) breakdown. Deleting the Mn2+-uptake system (MntH) causes a significant increase in the T6+ population, stipulating a deleterious effect on T6SS modulation in A. baumannii. Intriguingly, we identify a bonafide sRNA, AbsR28, that meditates the crosstalk between MntH and T6SS. This work elucidates a detailed mechanism of Mn2+-dependent AbsR28-mediated post-transcriptional repression of T6SS, exploited by A. baumannii to survive in the host and establish pathogenesis.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 30, 2022.
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Acinetobacter baumannii defends against oxidative stress through a Mn2+-dependent small RNA-mediated suppression of type VI secretion system
Somok Bhowmik, Avik Pathak, Kuldip Devnath, Tarun Kumar Sharma, Ramandeep Singh, Saurabh Chugh, Ranjana Pathania
bioRxiv 2022.07.30.502137; doi: https://doi.org/10.1101/2022.07.30.502137
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Acinetobacter baumannii defends against oxidative stress through a Mn2+-dependent small RNA-mediated suppression of type VI secretion system
Somok Bhowmik, Avik Pathak, Kuldip Devnath, Tarun Kumar Sharma, Ramandeep Singh, Saurabh Chugh, Ranjana Pathania
bioRxiv 2022.07.30.502137; doi: https://doi.org/10.1101/2022.07.30.502137

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