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Characterization of enhancer fragments in Drosophila robo2

Gina Hauptman, Marie C. Reichert, Muna A. Abdal Rhida, View ORCID ProfileTimothy A. Evans
doi: https://doi.org/10.1101/2022.08.01.502399
Gina Hauptman
1Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701
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Marie C. Reichert
1Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701
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Muna A. Abdal Rhida
1Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701
2Department of Biology, Wasit University, Iraq
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Timothy A. Evans
1Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701
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  • ORCID record for Timothy A. Evans
  • For correspondence: evanst@uark.edu
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Abstract

Receptor proteins of the Roundabout (Robo) family regulate axon guidance decisions during nervous system development. Among the three Drosophila robo family genes (robo1, robo2, and robo3), robo2 displays a dynamic expression pattern and regulates multiple axon guidance outcomes, including preventing midline crossing in some axons, promoting midline crossing in others, forming lateral longitudinal axon pathways, and regulating motor axon guidance. The identity and location of enhancer elements regulating robo2’s complex and dynamic expression pattern in different neural cell types are unknown. Here, we characterize a set of 17 transgenic lines expressing GAL4 under the control of DNA sequences derived from noncoding regions in and around robo2, to identify enhancers controlling specific aspects of robo2 expression in the embryonic ventral nerve cord. We identify individual fragments that confer expression in specific cell types where robo2 is known to function, including early pioneer neurons, midline glia, and lateral longitudinal neurons. Our results indicate that robo2’s dynamic expression pattern is specified by a combination of enhancer elements that are active in different subsets of cells. We show that robo2’s expression in lateral longitudinal axons represents two genetically separable subsets of neurons, and compare their axon projections with each other and with Fasciclin II (FasII), a commonly used marker of longitudinal axon pathways. In addition, we provide a general description of each fragment’s expression in embryonic tissues outside of the nervous system, to serve as a resource for other researchers who may be interested in robo2 expression and its functional roles outside the CNS.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 03, 2022.
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Characterization of enhancer fragments in Drosophila robo2
Gina Hauptman, Marie C. Reichert, Muna A. Abdal Rhida, Timothy A. Evans
bioRxiv 2022.08.01.502399; doi: https://doi.org/10.1101/2022.08.01.502399
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Characterization of enhancer fragments in Drosophila robo2
Gina Hauptman, Marie C. Reichert, Muna A. Abdal Rhida, Timothy A. Evans
bioRxiv 2022.08.01.502399; doi: https://doi.org/10.1101/2022.08.01.502399

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