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V2b neurons act via multiple targets in spinal motor networks

View ORCID ProfileMohini Sengupta, View ORCID ProfileMartha W. Bagnall
doi: https://doi.org/10.1101/2022.08.01.502410
Mohini Sengupta
1Washington University School of Medicine, Department of Neuroscience, St Louis, MO, USA
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Martha W. Bagnall
1Washington University School of Medicine, Department of Neuroscience, St Louis, MO, USA
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  • ORCID record for Martha W. Bagnall
  • For correspondence: bagnall@wustl.edu
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Abstract

Within the spinal cord, interneurons shape motor neuron activity. These interneurons can project over long distances in the longitudinal axis, but systematic mapping of their connectivity has been limited. In this study, using larval zebrafish, we mapped local and long-range connectivity of a cardinal spinal population, the Gata3+ V2b class. V2b neurons are inhibitory and project ipsilateral, descending axons. We show that V2b neurons are active during fictive swimming, slightly leading the motor burst. Via optogenetic mapping of output in the rostrocaudal axis, we demonstrate that V2b neurons robustly inhibit motor neurons and other major spinal interneuron classes, including V2a, V1, commissural neurons and other V2b neurons. V2b inhibition is patterned along the rostrocaudal axis, providing long-range inhibition to motor and V2a neurons but more localized innervation of the V1 class. Furthermore, these results provide the first demonstration of reciprocal V1/V2b inhibition in axial circuits, potentially representing an ancestral motif of the limb control network.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Include a new Supplemental figure.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 05, 2022.
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V2b neurons act via multiple targets in spinal motor networks
Mohini Sengupta, Martha W. Bagnall
bioRxiv 2022.08.01.502410; doi: https://doi.org/10.1101/2022.08.01.502410
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V2b neurons act via multiple targets in spinal motor networks
Mohini Sengupta, Martha W. Bagnall
bioRxiv 2022.08.01.502410; doi: https://doi.org/10.1101/2022.08.01.502410

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