Abstract
Three-dimensional electron-microscopy is an important imaging modality in contemporary cell biology. Identification of intracellular structures is laborious and time-consuming, however, and seriously impairs effective use of a potentially powerful tool. Resolving this bottleneck is therefore a critical next step in frontier biomedical imaging. We describe Automated Segmentation of intracellular substructures in Electron Microscopy (ASEM), a new pipeline to train a convolutional network to detect structures of wide range in size and complexity. We obtain for each structure a dedicated model based on a small number of sparsely annotated ground truth annotations from only one or two cells. To improve model generalization to different imaging conditions, we developed a rapid, computationally effective strategy to refine an already trained model by including a few additional annotations. We show the successful automated identification of mitochondria, Golgi apparatus, endoplasmic reticulum, nuclear pore complexes, clathrin coated pits and coated vesicles, and caveolae in cells imaged by focused ion beam scanning electron microscopy with quasi-isotropic resolution.
Summary Recent advances in automated segmentation using deep neural network models allow identification of subcellular structures. This study describes a new pipeline to train a convolutional network for rapid and efficient detection of structures of wide range in size and complexity.
Competing Interest Statement
The authors have declared no competing interest.
