Abstract
Highly multiplexed tissue imaging (MTI) are powerful spatial proteomics technologies that enable in situ single-cell characterization of tissues. However, analysis and visualization of MTI datasets remains challenging, and we developed the Galaxy-ME software hub to address this challenge.Galaxy-ME is a web-based, interactive software hub that enables end-to-end analysis and visualization of MTI datasets and is accessible to everyone. To demonstrate its utility, Galaxy-ME was used to analyze datasets obtained from multiple MTI assays and evaluate assay concordance in both normal and cancerous tissues. Galaxy-ME is a publicly available web resource.
Competing Interest Statement
J. Goecks has a significant financial interest in GalaxyWorks, a company that may have a commercial interest in the results of this research and technology. This competing interest is managed by OHSU, and Goecks declares that this relationship has not influenced the content of this manuscript. P.K. Sorger is a member of the SAB or BOD member of Applied Biomath, RareCyte Inc. and Glencoe Software, which distributes a commercial version of the OMERO database; P.K. Sorger is also a member of the NanoString SAB. In the last 5 years, the Sorger laboratory has received research funding from Novartis and Merck. Sorger declares that none of these relationships have influenced the content of this manuscript.
Footnotes
Results condensed, methods expanded. Revised Figures 2 and 3; supplementary figures added.
