Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Development of a multi-sensor integrated midbrain organoid-on-a-chip platform for studying Parkinson’s disease

Sarah Spitz, Silvia Bolognin, Konstanze Brandauer, Julia Füßl, Patrick Schuller, Silvia Schobesberger, Christian Jordan, Barbara Schädl, Johannes Grillari, Heinz D. Wanzenboeck, Torsten Mayr, Michael Harasek, Jens C. Schwamborn, Peter Ertl
doi: https://doi.org/10.1101/2022.08.19.504522
Sarah Spitz
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
hAustrian Cluster for Tissue Regeneration, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Silvia Bolognin
bDevelopmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, seven avenue des Hauts-Fourneaux, 4362 Esch-sur-Alzette, Luxembourg
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Konstanze Brandauer
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julia Füßl
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick Schuller
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Silvia Schobesberger
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christian Jordan
dInstitute of Chemical, Environmental and Bioscience Engineering, Thermal Process Engineering and Simulation, TU Wien, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Barbara Schädl
cLudwig Boltzmann Institute for Traumatology in Cooperation with AUVA, 1200 Vienna, Austria
gMedical University of Vienna, University Clinic of Dentistry; Vienna, Austria
hAustrian Cluster for Tissue Regeneration, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Johannes Grillari
cLudwig Boltzmann Institute for Traumatology in Cooperation with AUVA, 1200 Vienna, Austria
hAustrian Cluster for Tissue Regeneration, Vienna, Austria
iInstitute for Molecular Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heinz D. Wanzenboeck
eInstitute of Solid State Electronics, Vienna University of Technology, 1040 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Torsten Mayr
fInstitute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Harasek
dInstitute of Chemical, Environmental and Bioscience Engineering, Thermal Process Engineering and Simulation, TU Wien, 1060 Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jens C. Schwamborn
bDevelopmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, seven avenue des Hauts-Fourneaux, 4362 Esch-sur-Alzette, Luxembourg
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Ertl
aFaculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria
hAustrian Cluster for Tissue Regeneration, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: peter.ertl@tuwien.ac.at
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Due to its ability to recapitulate key pathological processes in vitro, midbrain organoid technology has significantly advanced the modeling of Parkinson’s disease over the last few years. However, some limitations such as insufficient tissue differentiation and maturation, deficient nutrient supply, and low analytical accessibility persist, altogether restricting the technology from reaching its full potential. To overcome these drawbacks, we have developed a multi-sensor integrated organ-on-a-chip platform capable of monitoring the electrophysiological, respiratory, and dopaminergic activity of human midbrain organoids. Our study showed that microfluidic cultivation resulted in a marked reduction in necrotic core formation, improved tissue differentiation as well as the recapitulation of key pathological hallmarks. Non-invasive monitoring employing an orthogonal sensing strategy revealed a clear time dependency in the onset of Parkinson’s disease-related phenotypes, reflecting the complex progression of the neurodegenerative disorder. Furthermore, drug-mediated rescue effects were observed after treatment with the repurposed compound 2-hydroxypropyl β-cyclodextrin, highlighting the platform’s potential in the context of drug screening applications as well as personalized medicine.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted August 22, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Development of a multi-sensor integrated midbrain organoid-on-a-chip platform for studying Parkinson’s disease
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Development of a multi-sensor integrated midbrain organoid-on-a-chip platform for studying Parkinson’s disease
Sarah Spitz, Silvia Bolognin, Konstanze Brandauer, Julia Füßl, Patrick Schuller, Silvia Schobesberger, Christian Jordan, Barbara Schädl, Johannes Grillari, Heinz D. Wanzenboeck, Torsten Mayr, Michael Harasek, Jens C. Schwamborn, Peter Ertl
bioRxiv 2022.08.19.504522; doi: https://doi.org/10.1101/2022.08.19.504522
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Development of a multi-sensor integrated midbrain organoid-on-a-chip platform for studying Parkinson’s disease
Sarah Spitz, Silvia Bolognin, Konstanze Brandauer, Julia Füßl, Patrick Schuller, Silvia Schobesberger, Christian Jordan, Barbara Schädl, Johannes Grillari, Heinz D. Wanzenboeck, Torsten Mayr, Michael Harasek, Jens C. Schwamborn, Peter Ertl
bioRxiv 2022.08.19.504522; doi: https://doi.org/10.1101/2022.08.19.504522

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (4655)
  • Biochemistry (10307)
  • Bioengineering (7618)
  • Bioinformatics (26200)
  • Biophysics (13453)
  • Cancer Biology (10625)
  • Cell Biology (15348)
  • Clinical Trials (138)
  • Developmental Biology (8455)
  • Ecology (12761)
  • Epidemiology (2067)
  • Evolutionary Biology (16777)
  • Genetics (11361)
  • Genomics (15405)
  • Immunology (10554)
  • Microbiology (25060)
  • Molecular Biology (10162)
  • Neuroscience (54128)
  • Paleontology (398)
  • Pathology (1655)
  • Pharmacology and Toxicology (2877)
  • Physiology (4314)
  • Plant Biology (9204)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6753)
  • Zoology (1453)