Abstract
Precision gene editing has been recently achieved by homology-directed gene targeting (HGT) and prime editing (PE), but implementation remains challenging in plants. Here, we report a novel tool for precision genome editing in plants employing microhomology-mediated end joining (MMEJ). The MMEJ-mediated precise gene replacement produced much higher targeted editing efficiencies than the cNHEJ, up to 8.89 %, 4.47 %, and 8.98 % in tomato, lettuce, and cabbage, respectively.
Competing Interest Statement
The authors have applied for Korean patents (10-2021-0089814) and PCT patents (PCT-KR2021-008727) based on the results reported in this paper.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted August 27, 2022.
CRISPR/Cas-based precision gene replacement in plants via homologous recombination-independent approaches
