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Conformational Changes Regulate Metal Coordination in the Catalytic Sites of Cas9

Anuska Das, Jay Rai, Mitchell O. Roth, Yuerong Shu, Megan L. Medina, MacKenzie R. Barakat, View ORCID ProfileHong Li
doi: https://doi.org/10.1101/2022.09.10.507422
Anuska Das
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
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Jay Rai
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
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Mitchell O. Roth
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
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Yuerong Shu
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
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Megan L. Medina
2Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
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MacKenzie R. Barakat
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
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Hong Li
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
2Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
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  • ORCID record for Hong Li
  • For correspondence: hong.li@fsu.edu
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Article Information

doi 
https://doi.org/10.1101/2022.09.10.507422
History 
  • September 11, 2022.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Anuska Das1,%,
  2. Jay Rai1,%,
  3. Mitchell O. Roth1,
  4. Yuerong Shu1,
  5. Megan L. Medina2,
  6. MacKenzie R. Barakat1 and
  7. Hong Li1,2,*
  1. 1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA
  2. 2Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
  1. ↵*Corresponding author: hong.li{at}fsu.edu
  1. ↵% Authors contributed equally.

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Posted September 11, 2022.
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Conformational Changes Regulate Metal Coordination in the Catalytic Sites of Cas9
Anuska Das, Jay Rai, Mitchell O. Roth, Yuerong Shu, Megan L. Medina, MacKenzie R. Barakat, Hong Li
bioRxiv 2022.09.10.507422; doi: https://doi.org/10.1101/2022.09.10.507422
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Conformational Changes Regulate Metal Coordination in the Catalytic Sites of Cas9
Anuska Das, Jay Rai, Mitchell O. Roth, Yuerong Shu, Megan L. Medina, MacKenzie R. Barakat, Hong Li
bioRxiv 2022.09.10.507422; doi: https://doi.org/10.1101/2022.09.10.507422

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