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Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of “Candidatus Accumulibacter”

Sergio Tomás-Martínez, Le Min Chen, Martin Pabst, David G. Weissbrodt, Mark C.M. van Loosdrecht, Yuemei Lin
doi: https://doi.org/10.1101/2022.09.16.508216
Sergio Tomás-Martínez
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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  • For correspondence: S.TomasMartinez@tudelft.nl
Le Min Chen
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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Martin Pabst
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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David G. Weissbrodt
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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Mark C.M. van Loosdrecht
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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Yuemei Lin
Department of Biotechnology, Delft University of Technology. Van der Maasweg 9,2629 HZ, Delft, The Netherlands
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Abstract

Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular polymeric substances (EPS). In order to employ a “Ca. Accumulibacter” enrichment as production platform for bacterial sialic acids, it is necessary to determine which fractions of the EPS of “Ca. Accumulibacter” contain NulOs and how to enrich and/or isolate them. We extracted the EPS from granules enriched with “Ca. Accumulibcater” and used size-exclusion chromatography to separate them into different molecular weight fractions. This separation resulted in two high molecular weight (> 5,500 kDa) fractions dominated by polysaccharides, with a NulO content up to 4 times higher than the extracted EPS. This suggests that NulOs in “Ca. Accumulibacter” are likely located in high molecular weight polysaccharides. Additionally, it was seen that the extracted EPS and the NulO-rich fractions can bind and neutralize histones. This suggest that they can serve as source for sepsis treatment drugs, although further purification needs to be evaluated.

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Highlights

  • NulOs in “Ca. Accumulibacter” are likely located in high molecular weight polysaccharides.

  • Size exclusion chromatography allows to obtain high molecular weight polysaccharide-rich fractions enriched with NulOs.

  • EPS and the NulOs-rich fractions can serve as source for sepsis treatment drugs.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 16, 2022.
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Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of “Candidatus Accumulibacter”
Sergio Tomás-Martínez, Le Min Chen, Martin Pabst, David G. Weissbrodt, Mark C.M. van Loosdrecht, Yuemei Lin
bioRxiv 2022.09.16.508216; doi: https://doi.org/10.1101/2022.09.16.508216
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Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of “Candidatus Accumulibacter”
Sergio Tomás-Martínez, Le Min Chen, Martin Pabst, David G. Weissbrodt, Mark C.M. van Loosdrecht, Yuemei Lin
bioRxiv 2022.09.16.508216; doi: https://doi.org/10.1101/2022.09.16.508216

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