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Chemical Imaging Reveals Diverse Functions of Tricarboxylic Acid Metabolites in Root Growth and Development

Tao Zhang, Sarah E. Noll, Jesus T. Peng, Amman Klair, Abigail Tripka, Nathan Stutzman, Casey Cheng, Richard N. Zare, Alexandra J. Dickinson
doi: https://doi.org/10.1101/2022.10.04.510836
Tao Zhang
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Sarah E. Noll
2Department of Chemistry, Stanford University, Stanford, CA 94305, USA
3Department of Chemistry, Pomona College, Claremont, CA 91711, USA
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Jesus T. Peng
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Amman Klair
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Abigail Tripka
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Nathan Stutzman
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Casey Cheng
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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Richard N. Zare
2Department of Chemistry, Stanford University, Stanford, CA 94305, USA
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  • For correspondence: zare@stanford.edu adickinson@ucsd.edu
Alexandra J. Dickinson
1Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA
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  • For correspondence: zare@stanford.edu adickinson@ucsd.edu
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Abstract

Understanding how plants grow is critical for agriculture and fundamental for illuminating principles of multicellular development 1. Here, we apply chemical mapping of the developing maize root using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) 2. This technique reveals a range of small molecule distribution patterns across the gradient of stem cell differentiation in the root. To understand the developmental logic of these patterns, we examined tricarboxylic acid (TCA) cycle metabolites. In both Arabidopsis and maize, TCA metabolites are enriched in developmentally opposing regions, suggesting that stem-cell specific TCA metabolite localization may be conserved in evolutionarily divergent species. We find that these metabolites, particularly succinate, aconitate, citrate, and α-ketoglutarate, control root development in diverse and distinct ways. Critically, the effects of metabolites on stem cell behavior can be independent of their canonical role in ATP production. These results present new insights into development and suggest practical means for controlling plant growth.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Competing Interest Statement: The authors declare no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 05, 2022.
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Chemical Imaging Reveals Diverse Functions of Tricarboxylic Acid Metabolites in Root Growth and Development
Tao Zhang, Sarah E. Noll, Jesus T. Peng, Amman Klair, Abigail Tripka, Nathan Stutzman, Casey Cheng, Richard N. Zare, Alexandra J. Dickinson
bioRxiv 2022.10.04.510836; doi: https://doi.org/10.1101/2022.10.04.510836
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Chemical Imaging Reveals Diverse Functions of Tricarboxylic Acid Metabolites in Root Growth and Development
Tao Zhang, Sarah E. Noll, Jesus T. Peng, Amman Klair, Abigail Tripka, Nathan Stutzman, Casey Cheng, Richard N. Zare, Alexandra J. Dickinson
bioRxiv 2022.10.04.510836; doi: https://doi.org/10.1101/2022.10.04.510836

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