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A cell size threshold triggers commitment to stomatal fate in Arabidopsis

View ORCID ProfileYan Gong, View ORCID ProfileRenee Dale, View ORCID ProfileHannah F. Fung, View ORCID ProfileGabriel O. Amador, View ORCID ProfileMargot E. Smit, View ORCID ProfileDominique C. Bergmann
doi: https://doi.org/10.1101/2022.10.09.510391
Yan Gong
1Department of Biology, Stanford University, Stanford, CA 94305, USA
5Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
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  • ORCID record for Yan Gong
Renee Dale
2Donald Danforth Plant Science Center, St. Louis, MO 63132 USA
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Hannah F. Fung
1Department of Biology, Stanford University, Stanford, CA 94305, USA
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Gabriel O. Amador
3Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
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Margot E. Smit
1Department of Biology, Stanford University, Stanford, CA 94305, USA
4Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
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Dominique C. Bergmann
1Department of Biology, Stanford University, Stanford, CA 94305, USA
4Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
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  • For correspondence: bergmann@stanford.edu
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Abstract

How flexible developmental programs integrate information from internal and external factors to modulate stem cell behavior is a fundamental question in developmental biology. Cells of the Arabidopsis stomatal lineage modify the balance of stem cell proliferation and differentiation to adjust the size and cell type composition of mature leaves. Here, we report that meristemoids, one type of stomatal lineage stem cell, trigger the transition from asymmetric self-renewing divisions to commitment and terminal differentiation by crossing a critical cell size threshold. Through computational simulation, we demonstrate that this cell size-mediated transition allows robust, yet flexible termination of stem cell proliferation and we observe adjustments in the number of divisions before the differentiation threshold under several genetic manipulations. We experimentally evaluate several mechanisms for cell size sensing, and our data suggest that cell size is sensed via a chromatin ruler acting in the nucleus.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 10, 2022.
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A cell size threshold triggers commitment to stomatal fate in Arabidopsis
Yan Gong, Renee Dale, Hannah F. Fung, Gabriel O. Amador, Margot E. Smit, Dominique C. Bergmann
bioRxiv 2022.10.09.510391; doi: https://doi.org/10.1101/2022.10.09.510391
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A cell size threshold triggers commitment to stomatal fate in Arabidopsis
Yan Gong, Renee Dale, Hannah F. Fung, Gabriel O. Amador, Margot E. Smit, Dominique C. Bergmann
bioRxiv 2022.10.09.510391; doi: https://doi.org/10.1101/2022.10.09.510391

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