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A gelatin-based feed for individually tailored drug delivery to adult zebrafish

View ORCID ProfileAleksander J. Ochocki, View ORCID ProfileJustin W. Kenney
doi: https://doi.org/10.1101/2022.10.11.511627
Aleksander J. Ochocki
Department of Biological Sciences, Wayne State University, Detroit, MI 48202
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Justin W. Kenney
Department of Biological Sciences, Wayne State University, Detroit, MI 48202
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  • For correspondence: jkenney9a@gmail.com
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Abstract

Current approaches to drug delivery in adult zebrafish have significant limitations such as need for confinement, anesthesia, and/or dosing that is not based on body weight. To circumvent these challenges, we developed a non-invasive gelatin-based feed that is easily pipette into individually tailored morsels according to weight. Our feed was readily eaten by zebrafish (< 1 minute) with minimal leaching of compound (< 5%) when placed in water. We used our feed to deliver an NMDAR antagonist (MK-801, 4 mg/kg) prior to exposure to a novel tank. Consistent with prior work, we found that MK-801 caused a decrease in predator-avoidance/anxiety-like behaviors. We also found that MK-801 increased locomotion in male fish, but not females. Our simple, easy to prepare, and individually tailored gelatin-based feed now brings pharmacological manipulations of adult zebrafish in line with best practices used in other vertebrate model organisms.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 14, 2022.
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A gelatin-based feed for individually tailored drug delivery to adult zebrafish
Aleksander J. Ochocki, Justin W. Kenney
bioRxiv 2022.10.11.511627; doi: https://doi.org/10.1101/2022.10.11.511627
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A gelatin-based feed for individually tailored drug delivery to adult zebrafish
Aleksander J. Ochocki, Justin W. Kenney
bioRxiv 2022.10.11.511627; doi: https://doi.org/10.1101/2022.10.11.511627

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