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Versatile and automated workflow for the analysis of oligodendroglial calcium signals in preclinical mouse models of myelin repair

View ORCID ProfileDorien A. Maas, View ORCID ProfileBlandine Manot-Saillet, View ORCID ProfilePhilippe Bun, Chloé Habermacher, Corinne Poilbout, View ORCID ProfileFilippo Rusconi, View ORCID ProfileMaria Cecilia Angulo
doi: https://doi.org/10.1101/2022.10.14.512256
Dorien A. Maas
1Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, “Team: Interactions between neurons and oligodendroglia in myelination and myelin repair”, F-75014 Paris, France
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Blandine Manot-Saillet
1Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, “Team: Interactions between neurons and oligodendroglia in myelination and myelin repair”, F-75014 Paris, France
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Philippe Bun
2Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, “NeurImag platform”, F-75014 Paris, France
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Chloé Habermacher
1Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, “Team: Interactions between neurons and oligodendroglia in myelination and myelin repair”, F-75014 Paris, France
6SynapCell, Bâtiment Synergy Zac Isiparc, F-38330 Saint Ismier, France
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Corinne Poilbout
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Filippo Rusconi
4IDEEV, GQE, Université Paris-Saclay, CNRS, INRAE, AgroParisTech, 12, route 128, F-91272 Gif-sur-Yvette, France
5INSERM, UMR-S 1138, Centre de Recherche des Cordeliers, France; Sorbonne Université-INSERM, France; PAPPSO, Universite Paris-Saclay, INRAE, CNRS, France
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Maria Cecilia Angulo
1Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, “Team: Interactions between neurons and oligodendroglia in myelination and myelin repair”, F-75014 Paris, France
3GHU PARIS psychiatrie & neurosciences, F-75014 Paris, France
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  • For correspondence: maria-cecilia.angulo@parisdescartes.fr
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Abstract

Intracellular Ca2+ signals of oligodendroglia, the myelin-forming cells of the central nervous system, regulate vital cellular processes including myelination. However, studies on oligodendroglia Ca2+ signal dynamics are still scarce, especially during myelin repair, and there are no software solutions to properly analyze the unique Ca2+ signal characteristics in these cells. Here, we provide a comprehensive experimental and analytical workflow to acquire and analyze Ca2+ imaging data of oligodendroglia at the population and single-cell levels in preclinical mouse models of myelin repair. We report diverse ex vivo and in vivo experimental protocols to obtain reproducible Ca2+ imaging data from oligodendroglia in demyelinated lesions. Importantly, we provide an analytical pipeline containing two free, open source and cross-platform software programs, Occam and post-prOccam, that enable the fully automated analysis of one- and two-photon Ca2+ imaging datasets from oligodendroglia obtained by either ex vivo or in vivo Ca2+ imaging techniques. This versatile and accessible experimental and analytical framework, which revealed significant but uncorrelated spontaneous Ca2+ activity in oligodendroglia inside demyelinated lesions, should facilitate the elucidation of Ca2+-mediated mechanisms underlying remyelination and therefore help to accelerate the development of therapeutic strategies for the many myelin-related disorders, such as multiple sclerosis.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://gitlab.com/d5674/occam

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 18, 2022.
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Versatile and automated workflow for the analysis of oligodendroglial calcium signals in preclinical mouse models of myelin repair
Dorien A. Maas, Blandine Manot-Saillet, Philippe Bun, Chloé Habermacher, Corinne Poilbout, Filippo Rusconi, Maria Cecilia Angulo
bioRxiv 2022.10.14.512256; doi: https://doi.org/10.1101/2022.10.14.512256
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Versatile and automated workflow for the analysis of oligodendroglial calcium signals in preclinical mouse models of myelin repair
Dorien A. Maas, Blandine Manot-Saillet, Philippe Bun, Chloé Habermacher, Corinne Poilbout, Filippo Rusconi, Maria Cecilia Angulo
bioRxiv 2022.10.14.512256; doi: https://doi.org/10.1101/2022.10.14.512256

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