Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Staphylococcus aureus FtsZ and PBP4 bind to the conformationally dynamic N-terminal domain of GpsB

Michael D. Sacco, Lauren R. Hammond, Radwan E. Noor, Dipanwita Bhattacharya, Jesper J. Madsen, Xiujun Zhang, Shane G. Butler, M. Trent Kemp, Aiden C. Jaskolka-Brown, Sebastian J. Khan, Ioannis Gelis, View ORCID ProfilePrahathees J. Eswara, View ORCID ProfileYu Chen
doi: https://doi.org/10.1101/2022.10.25.513704
Michael D. Sacco
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lauren R. Hammond
2Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Radwan E. Noor
3Department of Chemistry, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dipanwita Bhattacharya
2Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jesper J. Madsen
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
4Global and Planetary Health, College of Public Health, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiujun Zhang
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shane G. Butler
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Trent Kemp
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aiden C. Jaskolka-Brown
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian J. Khan
2Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ioannis Gelis
3Department of Chemistry, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Prahathees J. Eswara
2Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, 33620, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Prahathees J. Eswara
  • For correspondence: ychen1@usf.edu
Yu Chen
1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yu Chen
  • For correspondence: ychen1@usf.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Bacterial cell division is a tightly regulated process that requires the formation of a dynamic multi-protein complex. In the Firmicutes phylum, GpsB is a membrane associated protein that coordinates peptidoglycan synthesis for cell growth and division. Although GpsB has been studied in several organisms, the structure, function, and interactome of Staphylococcus aureus GpsB is largely uncharacterized, despite being reported as uniquely essential for growth in this clinically relevant bacterium. To address this knowledge gap, we solved the crystal structure of the N-terminal domain of S. aureus GpsB. This structure reveals an atypical asymmetric dimer, and major conformational flexibility that can be mapped to a hinge region formed by a three-residue insertion exclusive to Staphylococci. When this three-residue insertion is excised, its thermal stability increases, and the mutant no longer produces a previously reported lethal phenotype when overexpressed in Bacillus subtilis. Furthermore, we provide the first biochemical, biophysical, and crystallographic evidence that the N-terminal domain of GpsB binds not only PBP4, but also FtsZ, through a conserved recognition motif located on their C-terminus, thus linking peptidoglycan synthesis with cell division. Taken together, the unique structure of S. aureus GpsB and its direct interaction with FtsZ/PBP4 provide deeper insight into the central role of GpsB in S. aureus cell division.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 25, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Staphylococcus aureus FtsZ and PBP4 bind to the conformationally dynamic N-terminal domain of GpsB
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Staphylococcus aureus FtsZ and PBP4 bind to the conformationally dynamic N-terminal domain of GpsB
Michael D. Sacco, Lauren R. Hammond, Radwan E. Noor, Dipanwita Bhattacharya, Jesper J. Madsen, Xiujun Zhang, Shane G. Butler, M. Trent Kemp, Aiden C. Jaskolka-Brown, Sebastian J. Khan, Ioannis Gelis, Prahathees J. Eswara, Yu Chen
bioRxiv 2022.10.25.513704; doi: https://doi.org/10.1101/2022.10.25.513704
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Staphylococcus aureus FtsZ and PBP4 bind to the conformationally dynamic N-terminal domain of GpsB
Michael D. Sacco, Lauren R. Hammond, Radwan E. Noor, Dipanwita Bhattacharya, Jesper J. Madsen, Xiujun Zhang, Shane G. Butler, M. Trent Kemp, Aiden C. Jaskolka-Brown, Sebastian J. Khan, Ioannis Gelis, Prahathees J. Eswara, Yu Chen
bioRxiv 2022.10.25.513704; doi: https://doi.org/10.1101/2022.10.25.513704

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4232)
  • Biochemistry (9128)
  • Bioengineering (6774)
  • Bioinformatics (23989)
  • Biophysics (12117)
  • Cancer Biology (9522)
  • Cell Biology (13772)
  • Clinical Trials (138)
  • Developmental Biology (7627)
  • Ecology (11686)
  • Epidemiology (2066)
  • Evolutionary Biology (15504)
  • Genetics (10638)
  • Genomics (14322)
  • Immunology (9477)
  • Microbiology (22832)
  • Molecular Biology (9089)
  • Neuroscience (48954)
  • Paleontology (355)
  • Pathology (1480)
  • Pharmacology and Toxicology (2568)
  • Physiology (3844)
  • Plant Biology (8327)
  • Scientific Communication and Education (1471)
  • Synthetic Biology (2296)
  • Systems Biology (6186)
  • Zoology (1300)