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An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay

View ORCID ProfileRachel L Byrne, Susan Gould, View ORCID ProfileThomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, View ORCID ProfileGiancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
doi: https://doi.org/10.1101/2022.10.31.514483
Rachel L Byrne
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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  • For correspondence: rachel.byrne@lstmed.ac.uk
Susan Gould
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Thomas Edwards
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Dominic Wooding
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Barry Atkinson
2UK Health Security Agency, Porton Down, UK
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Ginny Moore
2UK Health Security Agency, Porton Down, UK
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Kieran Collings
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
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Cedric Boisdon
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
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Simon Maher
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
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Giancarlo Biagini
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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  • ORCID record for Giancarlo Biagini
Emily R Adams
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Tom Fletcher
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Shaun H Pennington
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
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Abstract

We present an optimised method for the recovery of laboratory generated SARS-CoV-2 virus by plaque assay. This method allows easy incorporation into existing standard operating procedures of biological containment level 3 (BCL3) laboratories.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 01, 2022.
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An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay
Rachel L Byrne, Susan Gould, Thomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, Giancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
bioRxiv 2022.10.31.514483; doi: https://doi.org/10.1101/2022.10.31.514483
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An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay
Rachel L Byrne, Susan Gould, Thomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, Giancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
bioRxiv 2022.10.31.514483; doi: https://doi.org/10.1101/2022.10.31.514483

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