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An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay
View ORCID ProfileRachel L Byrne, Susan Gould, View ORCID ProfileThomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, View ORCID ProfileGiancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
doi: https://doi.org/10.1101/2022.10.31.514483
Rachel L Byrne
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Susan Gould
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Thomas Edwards
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Dominic Wooding
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Barry Atkinson
2UK Health Security Agency, Porton Down, UK
Ginny Moore
2UK Health Security Agency, Porton Down, UK
Kieran Collings
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
Cedric Boisdon
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
Simon Maher
3Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK
Giancarlo Biagini
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Emily R Adams
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Tom Fletcher
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK
Shaun H Pennington
1Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK

Abstract
We present an optimised method for the recovery of laboratory generated SARS-CoV-2 virus by plaque assay. This method allows easy incorporation into existing standard operating procedures of biological containment level 3 (BCL3) laboratories.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted November 01, 2022.
An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay
Rachel L Byrne, Susan Gould, Thomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, Giancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
bioRxiv 2022.10.31.514483; doi: https://doi.org/10.1101/2022.10.31.514483
An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay
Rachel L Byrne, Susan Gould, Thomas Edwards, Dominic Wooding, Barry Atkinson, Ginny Moore, Kieran Collings, Cedric Boisdon, Simon Maher, Giancarlo Biagini, Emily R Adams, Tom Fletcher, Shaun H Pennington
bioRxiv 2022.10.31.514483; doi: https://doi.org/10.1101/2022.10.31.514483
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