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Uncovering the principles coordinating systems-level organelle biogenesis with cellular growth

Shixing Wang, Shankar Mukherji
doi: https://doi.org/10.1101/2022.11.01.514705
Shixing Wang
Department of Physics, Washington University in St. Louis, Department of Cell Biology & Physiology, Washington University School of Medicine
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Shankar Mukherji
Department of Physics, Washington University in St. Louis, Department of Cell Biology & Physiology, Washington University School of Medicine
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Abstract

Among the hallmark properties of the eukaryotic cell is its organization into specialized biochemical compartments known as organelles. Understanding how organelle biogenesis at systems-scale is coordinated with cellular growth rate and size is a major goal of quantitative cell biology. Here we map out the correlation structure of systems-level organelle biogenesis with cellular growth using “rainbow yeast”, a strain of Saccharomyces cerevisiae that expresses fluorescent labels for 6 major organelles. By carrying out hyperspectral imaging of thousands of single rainbow yeast cells, we decomposed the systems-level organelle biogenesis program into specific modes that characterize the response to changes in nutrient availability. Upon chemical biological dissection of this response, our results suggest that systems-level organelle biogenesis represents the sum of distinct organelle modes excited by growth rate and cell size separately. The flexibility afforded by this regulatory architecture may underlie how eukaryotic cells leverage compartmentalization to independently tune cell sizes and growth rates and satisfy potentially incompatible environmental and developmental constraints.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 02, 2022.
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Uncovering the principles coordinating systems-level organelle biogenesis with cellular growth
Shixing Wang, Shankar Mukherji
bioRxiv 2022.11.01.514705; doi: https://doi.org/10.1101/2022.11.01.514705
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Uncovering the principles coordinating systems-level organelle biogenesis with cellular growth
Shixing Wang, Shankar Mukherji
bioRxiv 2022.11.01.514705; doi: https://doi.org/10.1101/2022.11.01.514705

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