Abstract
We present an automated quad-channel patch-clamp technology platform for ex vivo brain slice electrophysiology, capable of both blind and two-photon targeted robotically automated patching. The robot scales up the patch clamp single cell recording technique to four simultaneous channels, with seal success rates for two-photon targeted and blind modes of 54% and 67% respectively. In 50% of targeted trials (where specific cells were required), at least 2 simultaneous recordings were obtained. For blind mode, most trials yielded dual or triple recordings. This robot, a milestone on the path to a true in vivo robotic multi-patching technology platform, will allow numerous studies into the function and connectivity patterns of both primary and secondary cell types.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
giv18{at}ic.ac.uk, poo15{at}ic.ac.uk
