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Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks

Baolei Yuan, Chongwei Bi, Jinchen Wang, Yiqing Jin, Khaled Alsayegh, Muhammad Tehseen, Gang Yi, Samir Hamdan, View ORCID ProfileYanyi Huang, View ORCID ProfileMo Li
doi: https://doi.org/10.1101/2022.11.16.516713
Baolei Yuan
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Chongwei Bi
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Jinchen Wang
2Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life sciences, Peking University, Beijing, China
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Yiqing Jin
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Khaled Alsayegh
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Muhammad Tehseen
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Gang Yi
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Samir Hamdan
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Yanyi Huang
2Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life sciences, Peking University, Beijing, China
3Institute for Cell Analysis, Shenzhen Bay laboratory, Shenzhen, China
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  • ORCID record for Yanyi Huang
Mo Li
1Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
4Bioengineering Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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  • ORCID record for Mo Li
  • For correspondence: mo.li@kaust.edu.sa
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Posted November 16, 2022.
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Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks
Baolei Yuan, Chongwei Bi, Jinchen Wang, Yiqing Jin, Khaled Alsayegh, Muhammad Tehseen, Gang Yi, Samir Hamdan, Yanyi Huang, Mo Li
bioRxiv 2022.11.16.516713; doi: https://doi.org/10.1101/2022.11.16.516713
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Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks
Baolei Yuan, Chongwei Bi, Jinchen Wang, Yiqing Jin, Khaled Alsayegh, Muhammad Tehseen, Gang Yi, Samir Hamdan, Yanyi Huang, Mo Li
bioRxiv 2022.11.16.516713; doi: https://doi.org/10.1101/2022.11.16.516713

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