Abstract
Background Darier, Hailey-Hailey, and Grover’s diseases are rare non-autoimmune acantholytic skin diseases. While these diseases have different underlying causes, they share defects in cell-cell adhesion in the epidermis and desmosome organization.
Objective To better understand the underlying mechanisms leading to disease in these conditions we performed RNA-seq on lesional skin samples from Darier, Hailey-Hailey, and Grover’s disease patients.
Methods RNA-seq and bioinformatics analyses were performed on banked paraffin embedded diagnostic samples from each disease. For detailed Methods, please see the Methods section in this article’s Online Repository at www.jacionline.org.
Results The transcriptomic profiles of Darier, Hailey-Hailey, and Grover’s disease were found to share a remarkable overlap, which did not extend to other common inflammatory skin diseases, psoriasis and atopic dermatitis. Analysis of enriched pathways showed a shared upregulation in keratinocyte differentiation and Th17 inflammatory pathways, and a decrease in cell adhesion and actin organization pathways in Darier, Hailey-Hailey, and Grover’s disease. Direct comparison to atopic dermatitis and psoriasis showed that the downregulation in actin organization pathways was a unique feature in Darier, Hailey-Hailey, and Grover’s disease.
Further, upstream regulator analysis suggests that a decrease in SRF/MRTF activity may be responsible for the downregulation of actin organization pathways. Staining for MRTFA in lesional skin samples showed a decrease in nuclear MRTFA in patient skin compared to normal skin.
Conclusion These findings highlight the significant level of similarity in the transcriptome of Darier, Hailey-Hailey, and Grover’s disease, and identify decreases in actin organization pathways as a unique signature present in these conditions.
Key Messages
Darier Disease, Hailey-Hailey Disease, and Grover’s Disease share similar transcriptional profiles suggesting common mechanisms of pathogenesis.
SRF/MRTFA activity is reduced in Darier Disease, Hailey-Hailey Disease and Grover’s disease, implicating actin organization in acantholysis.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
The authors declare that no conflict of interest exists
Funding: This work was supported by the National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases R01AR041836 and R01AR043380 to KJG, F32AR078645 to QRRC. ALP was supported by T32AR060710. LCT, JK, XX, PH, and JEG were supported by P30 AR075043. Histology services were provided by the Northwestern University Pathology Core supported by P30CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. This work was also supported by a generous donation from the Lee family. Further support was provided by the JL Mayberry endowment to KJG.
Abbreviations
- AD
- Atopic Dermatitis
- DD
- Darier Disease
- ELK1
- ETS transcription factor ELK1
- ELK3
- ETS transcription factor ELK3
- ELK4
- ETS transcription factor ELK4
- HHD
- Hailey-Hailey Disease
- IPA
- Ingenuity Pathway Analysis
- GD
- Grover’s Disease
- GSEA
- Gene Set Enrichment Analysis
- MRTFA
- Myocardin-related transcription factor A
- MRTFB
- Myocardin-related transcription factor B
- PCA
- Principle Component Analysis
- PSO
- Psoriasis
- SRF
- Serum response factor
- TAZ
- transcriptional coactivator with PDZ-binding motif
- YAP
- Yes-associated protein
