Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

New insights into the Germline Genes and CDR3 Repertoire of the TCRβ chain in Chiroptera

Hao Zhou, Jun Li, Dewei Zhou, Yingjie Wu, Xingliang Wang, Jiang Zhou, Qingqing Ma, Xinsheng Yao, Long Ma
doi: https://doi.org/10.1101/2022.12.05.519110
Hao Zhou
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Li
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dewei Zhou
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yingjie Wu
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xingliang Wang
2School of Life Sciences, Guizhou normal university, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiang Zhou
2School of Life Sciences, Guizhou normal university, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qingqing Ma
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xinsheng Yao
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: immunology@126.com 164849671@qq.com
Long Ma
1Department of Immunology, Center of Immunomolecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: immunology@126.com 164849671@qq.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Bats are known to be natural reservoirs of many viruses, yet their unique immune system enables them to coexist with viruses without frequently exhibiting disease symptoms. The current understanding of the bat adaptive immune system is limited, as there is no database or tool capable of processing T-cell receptor (TCR) sequences for bats, and the diverse nature of the Chiroptera. We established a reference library of TCR-β germline genes by annotating three Chiroptera: The Greater Horseshoe Bat (Rhinolophus ferrumequinum, R. ferrumequinum), The Pale spear-nosed Bat (Phyllostomus discolor, P. discolor), and the Common Pipistrelle (Pipistrellus pipistrellus, P. pipistrellus). The distinct variations in the distribution of TRBV genes among the three types of bats could have a direct impact on the diversity of the TCR repertoire, as evidenced by the presence of conserved amino acids that indicate the T-cell recognition of antigens in bats is MHC-restricted. Furthermore, we conducted an analysis of the TCR-β repertoire in the Intermediate Horseshoe Bat (Rhinolophus affinis, R. affinis) using high-throughput sequencing (HTS). The bats’ TCR-β repertoire is formed through rearrangement of the V-D-J-C genes, with D-J/V-D deletions and insertion resulting a high diversity.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Manuscript has been re-written.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted February 28, 2023.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
New insights into the Germline Genes and CDR3 Repertoire of the TCRβ chain in Chiroptera
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
New insights into the Germline Genes and CDR3 Repertoire of the TCRβ chain in Chiroptera
Hao Zhou, Jun Li, Dewei Zhou, Yingjie Wu, Xingliang Wang, Jiang Zhou, Qingqing Ma, Xinsheng Yao, Long Ma
bioRxiv 2022.12.05.519110; doi: https://doi.org/10.1101/2022.12.05.519110
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
New insights into the Germline Genes and CDR3 Repertoire of the TCRβ chain in Chiroptera
Hao Zhou, Jun Li, Dewei Zhou, Yingjie Wu, Xingliang Wang, Jiang Zhou, Qingqing Ma, Xinsheng Yao, Long Ma
bioRxiv 2022.12.05.519110; doi: https://doi.org/10.1101/2022.12.05.519110

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4384)
  • Biochemistry (9602)
  • Bioengineering (7098)
  • Bioinformatics (24882)
  • Biophysics (12625)
  • Cancer Biology (9964)
  • Cell Biology (14364)
  • Clinical Trials (138)
  • Developmental Biology (7964)
  • Ecology (12113)
  • Epidemiology (2067)
  • Evolutionary Biology (15994)
  • Genetics (10932)
  • Genomics (14745)
  • Immunology (9873)
  • Microbiology (23682)
  • Molecular Biology (9486)
  • Neuroscience (50901)
  • Paleontology (369)
  • Pathology (1540)
  • Pharmacology and Toxicology (2683)
  • Physiology (4022)
  • Plant Biology (8658)
  • Scientific Communication and Education (1510)
  • Synthetic Biology (2397)
  • Systems Biology (6442)
  • Zoology (1346)