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Plasmodesmal connectivity in C4 Gynandropsis gynandra is induced by light and dependent on photosynthesis

View ORCID ProfileTina B. Schreier, Karin H. Müller, View ORCID ProfileSimona Eicke, View ORCID ProfileChristine Faulkner, View ORCID ProfileSamuel C. Zeeman, View ORCID ProfileJulian M. Hibberd
doi: https://doi.org/10.1101/2022.12.07.519530
Tina B. Schreier
1Department of Plant Sciences, University of Cambridge, Downing Street, CB1 3EA Cambridge, United Kingdom
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  • For correspondence: tbs32@cam.ac.uk jmh65@cam.ac.uk
Karin H. Müller
2Cambridge Advanced Imaging Centre (CAIC), University of Cambridge, Downing Street, CB2 3DY Cambridge, United Kingdom
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Simona Eicke
3Institute of Molecular Plant Biology, ETH Zurich, CH-8092 Zurich, Switzerland
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Christine Faulkner
4Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
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Samuel C. Zeeman
3Institute of Molecular Plant Biology, ETH Zurich, CH-8092 Zurich, Switzerland
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Julian M. Hibberd
1Department of Plant Sciences, University of Cambridge, Downing Street, CB1 3EA Cambridge, United Kingdom
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  • For correspondence: tbs32@cam.ac.uk jmh65@cam.ac.uk
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SUMMARY

  • In leaves of C4 plants the reactions of photosynthesis become restricted between two compartments. Typically, this allows accumulation of C4 acids in mesophyll cells to drive their diffusion into the bundle sheath. In C4 monocotyledonous grasses proliferation of plasmodesmata between these cell types is thought to increase cell-to-cell connectivity to allow efficient metabolite movement. However, it is not clear if C4 dicotyledons also show enhanced plasmodesmal connectivity between these cell types and whether this is a general requirement for C4 photosynthesis is not known. How mesophyll and bundle sheath cells in C4 leaves become highly connected is also not known.

  • We investigated these questions using 3D- and 2D-electron microscopy on the C4 dicotyledon Gynandropsis gynandra, and phylogenetically close C3 relatives.

  • The mesophyll-bundle sheath interface of C4 G. gynandra showed higher plasmodesmal frequency compared with closely related C3 species. Formation of these plasmodesmata was induced by light. Pharmacological agents that perturbed chloroplast development or photosynthesis reduced the number of plasmodesmata, but this inhibitory effect could be reversed by the provision of exogenous sucrose.

  • We conclude that the enhanced plasmodesmata formation between mesophyll and bundle sheath cells of C4 G. gynandra appears to be wired to the induction of C4 photosynthesis.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted December 12, 2022.
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Plasmodesmal connectivity in C4 Gynandropsis gynandra is induced by light and dependent on photosynthesis
Tina B. Schreier, Karin H. Müller, Simona Eicke, Christine Faulkner, Samuel C. Zeeman, Julian M. Hibberd
bioRxiv 2022.12.07.519530; doi: https://doi.org/10.1101/2022.12.07.519530
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Plasmodesmal connectivity in C4 Gynandropsis gynandra is induced by light and dependent on photosynthesis
Tina B. Schreier, Karin H. Müller, Simona Eicke, Christine Faulkner, Samuel C. Zeeman, Julian M. Hibberd
bioRxiv 2022.12.07.519530; doi: https://doi.org/10.1101/2022.12.07.519530

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