Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing

Romina Piscitello-Gómez, View ORCID ProfileFranz S Gruber, View ORCID ProfileAbhijeet Krishna, View ORCID ProfileCharlie Duclut, View ORCID ProfileCarl D Modes, Marko Popović, View ORCID ProfileFrank Jülicher, View ORCID ProfileNatalie A Dye, View ORCID ProfileSuzanne Eaton
doi: https://doi.org/10.1101/2022.12.09.519799
Romina Piscitello-Gómez
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Franz S Gruber
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
3National Phenotypic Screening Centre, University of Dundee, Dundee, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Franz S Gruber
Abhijeet Krishna
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
4Center for Systems Biology Dresden, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Abhijeet Krishna
Charlie Duclut
5Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
6Université Paris Cité, Laboratoire Matière et Systèmes Complexes, Paris, France
7Laboratoire Physico-Chimie Curie, CNRS UMR 168, Institut Curie, Université PSL, Sorbonne Université, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Charlie Duclut
Carl D Modes
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
4Center for Systems Biology Dresden, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carl D Modes
Marko Popović
4Center for Systems Biology Dresden, Dresden, Germany
5Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Frank Jülicher
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
4Center for Systems Biology Dresden, Dresden, Germany
5Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Frank Jülicher
Natalie A Dye
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
8Mildred Scheel Nachwuchszentrum P2, Medical Faculty, Technische Universität Dresden, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Natalie A Dye
  • For correspondence: natalie_anne.dye@tu-dresden.de
Suzanne Eaton
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
2DFG Excellence Cluster Physics of Life, Technische Universität Dresden, Dresden, Germany
4Center for Systems Biology Dresden, Dresden, Germany
9Biotechnologisches Zentrum, Technische Universität Dresden, Dresden, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Suzanne Eaton
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. We showed previously (Etournay et al., 2015) that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned. The core planar cell polarity (PCP) pathway influences morphogenetic cell movements in many other contexts, which suggests that it could globally pattern active cellular behaviors during pupal wing morphogenesis. We show here, however, that this is not the case: there is no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation, they do not affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis are independent of one potential chemical guiding cue, core PCP.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵§ Deceased

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 10, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing
Romina Piscitello-Gómez, Franz S Gruber, Abhijeet Krishna, Charlie Duclut, Carl D Modes, Marko Popović, Frank Jülicher, Natalie A Dye, Suzanne Eaton
bioRxiv 2022.12.09.519799; doi: https://doi.org/10.1101/2022.12.09.519799
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing
Romina Piscitello-Gómez, Franz S Gruber, Abhijeet Krishna, Charlie Duclut, Carl D Modes, Marko Popović, Frank Jülicher, Natalie A Dye, Suzanne Eaton
bioRxiv 2022.12.09.519799; doi: https://doi.org/10.1101/2022.12.09.519799

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4222)
  • Biochemistry (9095)
  • Bioengineering (6733)
  • Bioinformatics (23916)
  • Biophysics (12066)
  • Cancer Biology (9484)
  • Cell Biology (13720)
  • Clinical Trials (138)
  • Developmental Biology (7614)
  • Ecology (11644)
  • Epidemiology (2066)
  • Evolutionary Biology (15459)
  • Genetics (10610)
  • Genomics (14281)
  • Immunology (9447)
  • Microbiology (22749)
  • Molecular Biology (9056)
  • Neuroscience (48811)
  • Paleontology (354)
  • Pathology (1478)
  • Pharmacology and Toxicology (2558)
  • Physiology (3817)
  • Plant Biology (8299)
  • Scientific Communication and Education (1466)
  • Synthetic Biology (2285)
  • Systems Biology (6163)
  • Zoology (1295)