New Results
sgRNA constraints and genetic limitations for efficient Cas9 genome editing to generate knock-outs
Irmgard U. Haussmann, Thomas C. Dix, David W. J. Mcquarrie, Veronica Dezi, Abdullah I. Hans, Roland Arnold, Matthias Soller
doi: https://doi.org/10.1101/2022.12.15.520550
Irmgard U. Haussmann
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
2College of Life Science, Birmingham City University, Birmingham, B5 3TN, United Kingdom
Thomas C. Dix
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
David W. J. Mcquarrie
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Veronica Dezi
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Abdullah I. Hans
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Roland Arnold
3Institute of Cancer and Genomics Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
4Birmingham Centre for Genome Biology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Matthias Soller
1School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
4Birmingham Centre for Genome Biology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom

Article usage
Posted March 25, 2024.
sgRNA constraints and genetic limitations for efficient Cas9 genome editing to generate knock-outs
Irmgard U. Haussmann, Thomas C. Dix, David W. J. Mcquarrie, Veronica Dezi, Abdullah I. Hans, Roland Arnold, Matthias Soller
bioRxiv 2022.12.15.520550; doi: https://doi.org/10.1101/2022.12.15.520550
Subject Area
Subject Areas
- Biochemistry (14121)
- Bioengineering (10790)
- Bioinformatics (34225)
- Biophysics (17608)
- Cancer Biology (14700)
- Cell Biology (20721)
- Clinical Trials (138)
- Developmental Biology (11155)
- Ecology (16470)
- Epidemiology (2067)
- Evolutionary Biology (20773)
- Genetics (13649)
- Genomics (19056)
- Immunology (14196)
- Microbiology (33066)
- Molecular Biology (13790)
- Neuroscience (72195)
- Paleontology (539)
- Pathology (2274)
- Pharmacology and Toxicology (3852)
- Physiology (6089)
- Plant Biology (12362)
- Synthetic Biology (3447)
- Systems Biology (8353)
- Zoology (1905)