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Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14

Ming Wang, Zhenhui Zhong, Javier Gallego-Bartolomé, Suhua Feng, Yuan-Hsin Shih, Mukun Liu, Jessica Zhou, John Curtis Richey, Charmaine Ng, Yasaman Jami-Alahmadi, James Wohlschlegel, Keqiang Wu, Steven E. Jacobsen
doi: https://doi.org/10.1101/2023.01.10.523456
Ming Wang
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Zhenhui Zhong
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Javier Gallego-Bartolomé
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
5Instituto de Biología Molecular y Celular de Plantas (IBMCP), CSIC-Universitat Politècnica de València, 46022 Valencia, Spain
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Suhua Feng
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
2Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Yuan-Hsin Shih
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
3Institute of Plant Biology, National Taiwan University, Taipei, 10617 Taiwan
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Mukun Liu
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Jessica Zhou
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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John Curtis Richey
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Charmaine Ng
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
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Yasaman Jami-Alahmadi
4Department of Biological Chemistry, University of California at Los Angeles, CA 90095, USA
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James Wohlschlegel
4Department of Biological Chemistry, University of California at Los Angeles, CA 90095, USA
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Keqiang Wu
3Institute of Plant Biology, National Taiwan University, Taipei, 10617 Taiwan
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Steven E. Jacobsen
1Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA
2Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California at Los Angeles, Los Angeles, CA 90095, USA
4Department of Biological Chemistry, University of California at Los Angeles, CA 90095, USA
6Howard Hughes Medical Institute (HHMI), University of California at Los Angeles, Los Angeles, CA 90095, USA
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  • For correspondence: jacobsen@ucla.edu
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Abstract

Arabidopsis telomeric repeat binding factors (TRBs) can bind telomeric DNA sequences to protect telomeres from degradation. TRBs can also recruit Polycomb Repressive Complex 2 (PRC2) to deposit tri-methylation of H3 lysine 27 (H3K27me3) over certain target loci. Here, we demonstrate that TRBs also associate and colocalize with JUMONJI14 (JMJ14) and trigger H3K4me3 demethylation at some loci. The trb1/2/3 triple mutant and the jmj14-1 mutant show an increased level of H3K4me3 over TRB and JMJ14 binding sites, resulting in up-regulation of their target genes. Furthermore, tethering TRBs to the promoter region of genes with an artificial zinc finger (TRB-ZF) successfully triggers target gene silencing, as well as H3K27me3 deposition, and H3K4me3 removal. Interestingly, JMJ14 is predominantly recruited to ZF off-target sites with low levels of H3K4me3, which is accompanied with TRB-ZFs triggered H3K4me3 removal at these loci. These results suggest that TRB proteins coordinate PRC2 and JMJ14 activities to repress target genes via H3K27me3 deposition and H3K4me3 removal.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 10, 2023.
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Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14
Ming Wang, Zhenhui Zhong, Javier Gallego-Bartolomé, Suhua Feng, Yuan-Hsin Shih, Mukun Liu, Jessica Zhou, John Curtis Richey, Charmaine Ng, Yasaman Jami-Alahmadi, James Wohlschlegel, Keqiang Wu, Steven E. Jacobsen
bioRxiv 2023.01.10.523456; doi: https://doi.org/10.1101/2023.01.10.523456
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Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14
Ming Wang, Zhenhui Zhong, Javier Gallego-Bartolomé, Suhua Feng, Yuan-Hsin Shih, Mukun Liu, Jessica Zhou, John Curtis Richey, Charmaine Ng, Yasaman Jami-Alahmadi, James Wohlschlegel, Keqiang Wu, Steven E. Jacobsen
bioRxiv 2023.01.10.523456; doi: https://doi.org/10.1101/2023.01.10.523456

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