Oligonucleotide-directed proximity-interactome mapping (O-MAP): A unified method for discovering RNA-interacting proteins, transcripts and genomic loci in situ

Ashley F. Tsue; Evan E. Kania; Diana Q. Lei; Rose Fields; Christopher D. McGann; Elliot Hershberg; Xinxian Deng; Maryanne Kihiu; Shao-En Ong; Christine M. Disteche; Sita Kugel; Brian J. Beliveau; Devin K. Schweppe; David M. Shechner

doi:10.1101/2023.01.19.524825

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Oligonucleotide-directed proximity-interactome mapping (O-MAP): A unified method for discovering RNA-interacting proteins, transcripts and genomic loci in situ

View ORCID ProfileAshley F. Tsue, View ORCID ProfileEvan E. Kania, View ORCID ProfileDiana Q. Lei, Rose Fields, Christopher D. McGann, Elliot Hershberg, Xinxian Deng, View ORCID ProfileMaryanne Kihiu, View ORCID ProfileShao-En Ong, View ORCID ProfileChristine M. Disteche, View ORCID ProfileSita Kugel, View ORCID ProfileBrian J. Beliveau, View ORCID ProfileDevin K. Schweppe, View ORCID ProfileDavid M. Shechner

doi: https://doi.org/10.1101/2023.01.19.524825

Ashley F. Tsue

1Department of Pharmacology, University of Washington, Seattle, WA, USA

2Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

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Evan E. Kania

1Department of Pharmacology, University of Washington, Seattle, WA, USA

2Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

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Diana Q. Lei

1Department of Pharmacology, University of Washington, Seattle, WA, USA

2Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA

8Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

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Rose Fields

4Department of Genome Sciences, University of Washington, Seattle, WA, USA

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Christopher D. McGann

4Department of Genome Sciences, University of Washington, Seattle, WA, USA

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Elliot Hershberg

4Department of Genome Sciences, University of Washington, Seattle, WA, USA

9Department of Genetics, Stanford University, Stanford, CA, USA

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Xinxian Deng

5Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA

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Maryanne Kihiu

1Department of Pharmacology, University of Washington, Seattle, WA, USA

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Shao-En Ong

1Department of Pharmacology, University of Washington, Seattle, WA, USA

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Christine M. Disteche

5Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA

6Department of Medicine, University of Washington, Seattle, WA, USA

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Sita Kugel

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

7Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

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Brian J. Beliveau

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

4Department of Genome Sciences, University of Washington, Seattle, WA, USA

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Devin K. Schweppe

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

4Department of Genome Sciences, University of Washington, Seattle, WA, USA

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David M. Shechner

1Department of Pharmacology, University of Washington, Seattle, WA, USA

2Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA

3Brotman Baty Institute for Precision Medicine, Seattle, WA, USA

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For correspondence: shechner@uw.edu

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Posted January 20, 2023.

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Oligonucleotide-directed proximity-interactome mapping (O-MAP): A unified method for discovering RNA-interacting proteins, transcripts and genomic loci in situ

Ashley F. Tsue, Evan E. Kania, Diana Q. Lei, Rose Fields, Christopher D. McGann, Elliot Hershberg, Xinxian Deng, Maryanne Kihiu, Shao-En Ong, Christine M. Disteche, Sita Kugel, Brian J. Beliveau, Devin K. Schweppe, David M. Shechner

bioRxiv 2023.01.19.524825; doi: https://doi.org/10.1101/2023.01.19.524825

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Oligonucleotide-directed proximity-interactome mapping (O-MAP): A unified method for discovering RNA-interacting proteins, transcripts and genomic loci in situ

bioRxiv 2023.01.19.524825; doi: https://doi.org/10.1101/2023.01.19.524825

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