Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Functional Trajectories during innate spinal cord repair

Nicholas O. Jensen, Brooke Burris, Lili Zhou, Hunter Yamada, Catrina Reyes, View ORCID ProfileMayssa H. Mokalled
doi: https://doi.org/10.1101/2023.01.31.526502
Nicholas O. Jensen
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brooke Burris
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lili Zhou
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hunter Yamada
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catrina Reyes
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mayssa H. Mokalled
1Department of Developmental Biology Washington University School of Medicine, St. Louis, MO, USA
2Center of Regenerative Medicine Washington University School of Medicine, St. Louis, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mayssa H. Mokalled
  • For correspondence: mmokalled@wustl.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Adult zebrafish are capable of anatomical and functional recovery following severe spinal cord injury. Axon growth, glial bridging and adult neurogenesis are hallmarks of cellular regeneration during spinal cord repair. However, the correlation between these cellular regenerative processes and functional recovery remains to be elucidated. Whereas the majority of established functional regeneration metrics measure swim capacity, we hypothesize that gait quality is more directly related to neurological health. Here, we performed a longitudinal swim tracking study for sixty individual zebrafish spanning eight weeks of spinal cord regeneration. Multiple swim parameters as well as axonal and glial bridging were integrated. We established rostral compensation as a new gait quality metric that highly correlates with functional recovery. Tensor component analysis of longitudinal data supports a correspondence between functional recovery trajectories and neurological outcomes. Moreover, our studies predicted and validated that a subset of functional regeneration parameters measured 1 to 2 weeks post-injury is sufficient to predict the regenerative outcomes of individual animals at 8 weeks post-injury. Our findings established new functional regeneration parameters and generated a comprehensive correlative database between various functional and cellular regeneration outputs.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • title edit

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted February 01, 2023.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Functional Trajectories during innate spinal cord repair
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Functional Trajectories during innate spinal cord repair
Nicholas O. Jensen, Brooke Burris, Lili Zhou, Hunter Yamada, Catrina Reyes, Mayssa H. Mokalled
bioRxiv 2023.01.31.526502; doi: https://doi.org/10.1101/2023.01.31.526502
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Functional Trajectories during innate spinal cord repair
Nicholas O. Jensen, Brooke Burris, Lili Zhou, Hunter Yamada, Catrina Reyes, Mayssa H. Mokalled
bioRxiv 2023.01.31.526502; doi: https://doi.org/10.1101/2023.01.31.526502

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4234)
  • Biochemistry (9128)
  • Bioengineering (6774)
  • Bioinformatics (23989)
  • Biophysics (12117)
  • Cancer Biology (9523)
  • Cell Biology (13773)
  • Clinical Trials (138)
  • Developmental Biology (7627)
  • Ecology (11686)
  • Epidemiology (2066)
  • Evolutionary Biology (15506)
  • Genetics (10638)
  • Genomics (14322)
  • Immunology (9479)
  • Microbiology (22832)
  • Molecular Biology (9089)
  • Neuroscience (48987)
  • Paleontology (355)
  • Pathology (1480)
  • Pharmacology and Toxicology (2568)
  • Physiology (3844)
  • Plant Biology (8327)
  • Scientific Communication and Education (1471)
  • Synthetic Biology (2296)
  • Systems Biology (6187)
  • Zoology (1300)