Abstract
Single-cell atlases across conditions are essential in the characterization of human disease. In these complex experimental designs, patient samples are profiled across distinct cell-types and clinical conditions to describe disease processes at the cellular level. However, most of the current analysis tools are limited to pairwise cross-condition comparisons, disregarding the multicellular nature of disease processes and the effects of other biological and technical factors in the variation of gene expression. Here we propose a computational framework for an unsupervised analysis of samples from cross-condition single-cell atlases and for the identification of multicellular programs associated with disease. Our strategy, that repurposes multi-omics factor analysis, incorporates the variation of patient samples across cell-types and enables the joint analysis of multiple patient cohorts, facilitating integration of atlases. We applied our analysis to a collection of acute and chronic human heart failure single-cell datasets and described multicellular processes of cardiac remodeling that were conserved in independent spatial and bulk transcriptomics datasets. In sum, our framework serves as an exploratory tool for unsupervised analysis of cross-condition single-cell atlas and allows for the integration of the measurements of patient cohorts across distinct data modalities, facilitating the generation of comprehensive tissue-centric understanding of disease.
Competing Interest Statement
J.S.R. reports funding from GSK, Pfizer and Sanofi and fees from Travere Therapeutics, and Astex.
