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Inhibition of broomrape germination by 2,4-diacetylphloroglucinol produced by environmental Pseudomonas

View ORCID ProfileTristan Lurthy, Ségolène Perot, Florence Gerin-Eveillard, View ORCID ProfileMarjolaine Rey, View ORCID ProfileFlorence Wisniewski-Dyé, View ORCID ProfileJordan Vacheron, View ORCID ProfileClaire Prigent-Combaret
doi: https://doi.org/10.1101/2023.03.01.529533
Tristan Lurthy
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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  • For correspondence: tristan.lurthy@univ-lyon1.fr Jordan.Vacheron@unil.ch claire.prigent-combaret@univ-lyon1.fr
Ségolène Perot
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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Florence Gerin-Eveillard
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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Marjolaine Rey
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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Florence Wisniewski-Dyé
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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Jordan Vacheron
2Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
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  • For correspondence: tristan.lurthy@univ-lyon1.fr Jordan.Vacheron@unil.ch claire.prigent-combaret@univ-lyon1.fr
Claire Prigent-Combaret
1Ecologie Microbienne, Université Claude Bernard Lyon1, Université de Lyon, CNRS UMR-5557, INRAe UMR-1418, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
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  • For correspondence: tristan.lurthy@univ-lyon1.fr Jordan.Vacheron@unil.ch claire.prigent-combaret@univ-lyon1.fr
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Abstract

Phloroglucinol compounds (PGCs) produced by environmental Pseudomonas are well known for their capacity to limit plant-pathogen infection. Although PGCs and more specifically 2,4-diacetylphloroglucinol (DAPG) are well studied for their antimicrobial properties, they are to some extent toxic for crop plants. Parasitic weeds such as broomrapes (Phelipanche ramosa and Orobanche cumana) cause severe damage to crops and their development must be controlled. Here, we assessed the potential herbicidal effect of the bacterial model Pseudomonas ogarae F113, a PGCs-producing bacterium, on parasitic weed germination. We show using a mutagenesis approach that PGCs produced in bacterial supernatants are the main determinant inhibiting the germination of broomrapes. The use of individual or cocktails of pure PGCs revealed that the inhibition of the germination depends on the PGCs molecular structure and their concentrations as well as the broomrape species and pathovars. Furthermore, the inhibition caused by the PGCs is irreversible, causing a brown coloration of the broomrape seeds. Then, we evaluated in non-sterile soils the ability of bacterial inoculants or chemical DAPG to limit the infection of broomrapes on oil seed rape. Only the inoculation of PGCs-producing bacteria limited the infection of P. ramosa. Moreover, elemental profiling analysis of oil seed rape revealed that neither the inoculant nor applied DAPG affected the nutrition capacity of the oil seed rape. Our study expands the knowledge on the role that these multi-talented plant-beneficial Pseudomonas play in the environment and open new avenues for the development of natural bioherbicides to ward off parasitic plant infection.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* : co-senior authors

  • Conflict of interest: The authors declare no conflict of interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 01, 2023.
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Inhibition of broomrape germination by 2,4-diacetylphloroglucinol produced by environmental Pseudomonas
Tristan Lurthy, Ségolène Perot, Florence Gerin-Eveillard, Marjolaine Rey, Florence Wisniewski-Dyé, Jordan Vacheron, Claire Prigent-Combaret
bioRxiv 2023.03.01.529533; doi: https://doi.org/10.1101/2023.03.01.529533
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Inhibition of broomrape germination by 2,4-diacetylphloroglucinol produced by environmental Pseudomonas
Tristan Lurthy, Ségolène Perot, Florence Gerin-Eveillard, Marjolaine Rey, Florence Wisniewski-Dyé, Jordan Vacheron, Claire Prigent-Combaret
bioRxiv 2023.03.01.529533; doi: https://doi.org/10.1101/2023.03.01.529533

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