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Ultra-fast micro-CT of an unrestrained live insect

Leonidas-Romanos Davranoglou, Beth Mortimer, Christian Matthias Schlepütz, View ORCID ProfileGraham K. Taylor
doi: https://doi.org/10.1101/2023.03.03.531017
Leonidas-Romanos Davranoglou
1Oxford University Museum of Natural History, University of Oxford, Parks Road, Oxford OX1 3PW, UK
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  • For correspondence: leonidas-romanos.davranoglou@oum.ox.ac.uk
Beth Mortimer
3Department of Biology, Oxford University, Oxford OX1 3SZ, UK
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Christian Matthias Schlepütz
2TOMCAT beamline, Swiss Light Source, Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen PSI, Switzerland
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Graham K. Taylor
3Department of Biology, Oxford University, Oxford OX1 3SZ, UK
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  • ORCID record for Graham K. Taylor
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Abstract

Micro-CT has revolutionized functional morphology by enabling volumetric reconstruction of biological specimens at micrometre scales, but its accuracy is compromised by fixation artefacts. State-of-the-art in vivo imaging avoids this, but still requires subjects to be tethered, anaesthetized, or stained. Here we use ultra-fast synchrotron-based micro-CT to produce the first 3D scan of an unrestrained living organism at micrometre resolution, demonstrating the potential of this method in physiology, behaviour, and biomechanics.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 06, 2023.
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Ultra-fast micro-CT of an unrestrained live insect
Leonidas-Romanos Davranoglou, Beth Mortimer, Christian Matthias Schlepütz, Graham K. Taylor
bioRxiv 2023.03.03.531017; doi: https://doi.org/10.1101/2023.03.03.531017
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Ultra-fast micro-CT of an unrestrained live insect
Leonidas-Romanos Davranoglou, Beth Mortimer, Christian Matthias Schlepütz, Graham K. Taylor
bioRxiv 2023.03.03.531017; doi: https://doi.org/10.1101/2023.03.03.531017

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