Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans

View ORCID ProfileM. Caron, View ORCID ProfileV. Robert, L. Gely, View ORCID ProfileA. Adrait, View ORCID ProfileV. Pakulska, View ORCID ProfileY. Couté, View ORCID ProfileM. Chevalier, View ORCID ProfileC.G. Riedel, View ORCID ProfileC. Bedet, View ORCID ProfileF. Palladino
doi: https://doi.org/10.1101/2023.03.07.531480
M. Caron
1Laboratory of Biology and Modeling of the Cell, UMR5239 CNRS/Ecole Normale Supérieure de Lyon, INSERM U1210, UMS 3444 Biosciences Lyon Gerland, Université de Lyon, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for M. Caron
V. Robert
1Laboratory of Biology and Modeling of the Cell, UMR5239 CNRS/Ecole Normale Supérieure de Lyon, INSERM U1210, UMS 3444 Biosciences Lyon Gerland, Université de Lyon, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for V. Robert
L. Gely
1Laboratory of Biology and Modeling of the Cell, UMR5239 CNRS/Ecole Normale Supérieure de Lyon, INSERM U1210, UMS 3444 Biosciences Lyon Gerland, Université de Lyon, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Adrait
2Grenoble Alpes, CEA, Inserm, UA13 BGE, CNRS, CEA, FR2048, 38000 Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for A. Adrait
V. Pakulska
2Grenoble Alpes, CEA, Inserm, UA13 BGE, CNRS, CEA, FR2048, 38000 Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for V. Pakulska
Y. Couté
2Grenoble Alpes, CEA, Inserm, UA13 BGE, CNRS, CEA, FR2048, 38000 Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Y. Couté
M. Chevalier
3Department of Biosciences and Nutrition, Karolinska Institutet, Blickagången 16, 14157 Huddinge
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for M. Chevalier
C.G. Riedel
3Department of Biosciences and Nutrition, Karolinska Institutet, Blickagången 16, 14157 Huddinge
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for C.G. Riedel
C. Bedet
1Laboratory of Biology and Modeling of the Cell, UMR5239 CNRS/Ecole Normale Supérieure de Lyon, INSERM U1210, UMS 3444 Biosciences Lyon Gerland, Université de Lyon, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for C. Bedet
  • For correspondence: cecile.bedet@ens-lyon.fr
F. Palladino
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for F. Palladino
  • For correspondence: cecile.bedet@ens-lyon.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The SIN3 transcriptional coregulator influences gene expression through multiple interactions that include histone deacetylases (HDACs). Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability (ID)/autism syndromes, emphasizing its key role in development. However, little is known about the diversity of its interactions and functions in developmental processes. Here we show that loss of SIN-3, the single SIN3 homologue in Caenorhabditis elegans, results in maternal effect sterility associated with deregulation of the germline transcriptome, including desilencing of X-linked genes. We identify at least two distinct SIN3 complexes containing specific HDACs, and show that they differentially contribute to fertility. Single cell smFISH reveals that in sin-3 mutants, the X chromosome becomes re-expressed prematurely and in a stochastic manner in individual germ cells. Furthermore, we identify histone residues whose acetylation increases in the absence of SIN3. Together, this work provides a powerful framework for the in vivo study of SIN3 and associated proteins.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted March 08, 2023.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans
M. Caron, V. Robert, L. Gely, A. Adrait, V. Pakulska, Y. Couté, M. Chevalier, C.G. Riedel, C. Bedet, F. Palladino
bioRxiv 2023.03.07.531480; doi: https://doi.org/10.1101/2023.03.07.531480
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans
M. Caron, V. Robert, L. Gely, A. Adrait, V. Pakulska, Y. Couté, M. Chevalier, C.G. Riedel, C. Bedet, F. Palladino
bioRxiv 2023.03.07.531480; doi: https://doi.org/10.1101/2023.03.07.531480

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4684)
  • Biochemistry (10361)
  • Bioengineering (7682)
  • Bioinformatics (26340)
  • Biophysics (13534)
  • Cancer Biology (10692)
  • Cell Biology (15445)
  • Clinical Trials (138)
  • Developmental Biology (8501)
  • Ecology (12824)
  • Epidemiology (2067)
  • Evolutionary Biology (16867)
  • Genetics (11401)
  • Genomics (15484)
  • Immunology (10619)
  • Microbiology (25224)
  • Molecular Biology (10225)
  • Neuroscience (54481)
  • Paleontology (402)
  • Pathology (1669)
  • Pharmacology and Toxicology (2897)
  • Physiology (4345)
  • Plant Biology (9252)
  • Scientific Communication and Education (1586)
  • Synthetic Biology (2558)
  • Systems Biology (6781)
  • Zoology (1466)