A nanobody-based strategy for rapid and scalable purification of native human protein complexes

Taylor Anthony Stevens; Giovani Pinton Tomaleri; Masami Hazu; Sophia Wei; Vy N. Nguyen; Charlene DeKalb; Rebecca M. Voorhees; Tino Pleiner

doi:10.1101/2023.03.09.531980

New Results

A nanobody-based strategy for rapid and scalable purification of native human protein complexes

View ORCID ProfileTaylor Anthony Stevens, View ORCID ProfileGiovani Pinton Tomaleri, View ORCID ProfileMasami Hazu, Sophia Wei, View ORCID ProfileVy N. Nguyen, Charlene DeKalb, View ORCID ProfileRebecca M. Voorhees, View ORCID ProfileTino Pleiner

doi: https://doi.org/10.1101/2023.03.09.531980

Taylor Anthony Stevens

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Taylor Anthony Stevens

Giovani Pinton Tomaleri

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Giovani Pinton Tomaleri

Masami Hazu

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Masami Hazu

Sophia Wei

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Vy N. Nguyen

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Vy N. Nguyen

Charlene DeKalb

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Rebecca M. Voorhees

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Rebecca M. Voorhees
For correspondence: voorhees@caltech.edu pleiner@stanford.edu

Tino Pleiner

1Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Tino Pleiner
For correspondence: voorhees@caltech.edu pleiner@stanford.edu

Abstract
Full Text
Info/History
Metrics
Supplementary material
Preview PDF

Article usage

Article usage: March 2023 to May 2023

	Abstract	Full	Pdf
Mar 2023	1818	87	664
Apr 2023	325	54	160
May 2023	215	36	130

View the discussion thread.

PreviousNext

Posted March 10, 2023.

Download PDF

Supplementary Material

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Your Email *

Your Name *

Send To *

Enter multiple addresses on separate lines or separate them with commas.

You are going to email the following A nanobody-based strategy for rapid and scalable purification of native human protein complexes

Message Subject (Your Name) has forwarded a page to you from bioRxiv

Message Body (Your Name) thought you would like to see this page from the bioRxiv website.

Your Personal Message

CAPTCHA

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

A nanobody-based strategy for rapid and scalable purification of native human protein complexes

Taylor Anthony Stevens, Giovani Pinton Tomaleri, Masami Hazu, Sophia Wei, Vy N. Nguyen, Charlene DeKalb, Rebecca M. Voorhees, Tino Pleiner

bioRxiv 2023.03.09.531980; doi: https://doi.org/10.1101/2023.03.09.531980

Share This Article:

Citation Tools

A nanobody-based strategy for rapid and scalable purification of native human protein complexes

Taylor Anthony Stevens, Giovani Pinton Tomaleri, Masami Hazu, Sophia Wei, Vy N. Nguyen, Charlene DeKalb, Rebecca M. Voorhees, Tino Pleiner

bioRxiv 2023.03.09.531980; doi: https://doi.org/10.1101/2023.03.09.531980

Citation Manager Formats

BibTeX
Bookends
EasyBib
EndNote (tagged)
EndNote 8 (xml)
Medlars
Mendeley
Papers
RefWorks Tagged
Ref Manager
RIS
Zotero

Tweet Widget
Facebook Like
Google Plus One

Subject Area

Biochemistry

Subject Areas

All Articles

Animal Behavior and Cognition (4382)
Biochemistry (9591)
Bioengineering (7090)
Bioinformatics (24856)
Biophysics (12600)
Cancer Biology (9955)
Cell Biology (14349)
Clinical Trials (138)
Developmental Biology (7948)
Ecology (12105)
Epidemiology (2067)
Evolutionary Biology (15988)
Genetics (10925)
Genomics (14738)
Immunology (9869)
Microbiology (23659)
Molecular Biology (9484)
Neuroscience (50855)
Paleontology (369)
Pathology (1539)
Pharmacology and Toxicology (2681)
Physiology (4013)
Plant Biology (8657)
Scientific Communication and Education (1508)
Synthetic Biology (2394)
Systems Biology (6433)
Zoology (1346)