Abstract
A rapid, efficient 3D-resolved hyperspectral microscope is presented. Able to switch between hyperspectral and normal imaging modes in seconds without realignment, it is built around a commercial inverted microscope frame for ease-of-use. Performance is demonstrated on a variety of samples; utility is shown by performing highly-multiplexed fluorescence microscopy, and the sensitivity and resolution is highlighted by rapidly taking spontaneous Raman maps of micro-plastics and zebrafish embryos.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Posted March 16, 2023.
