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Simple and highly efficient detection of PSD95 using a nanobody and its recombinant minibody derivative

Markus Kilisch, Maja Gere-Becker, Liane Wüstefeld, Christel Bonnas, Alexander Crauel, Maja Mechmershausen, Henrik Martens, Hansjörg Götzke, View ORCID ProfileFelipe Opazo, Steffen Frey
doi: https://doi.org/10.1101/2023.03.16.532938
Markus Kilisch
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Maja Gere-Becker
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Liane Wüstefeld
2Synaptic Systems GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Christel Bonnas
2Synaptic Systems GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Alexander Crauel
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Maja Mechmershausen
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Henrik Martens
2Synaptic Systems GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Hansjörg Götzke
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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Felipe Opazo
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
3Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073 Göttingen, Germany
4Center for Biostructural Imaging of Neurodegeneration (BIN), University of Göttingen Medical Center, 37075 Göttingen, Germany
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  • ORCID record for Felipe Opazo
  • For correspondence: felipe.opazo@nano-tag.com steffen.frey@nano-tag.com
Steffen Frey
1NanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen
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  • For correspondence: felipe.opazo@nano-tag.com steffen.frey@nano-tag.com
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Abstract

The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has been implicated with several neurological disorders, making it an attractive target to develop strategies able to monitor PSD95 accurately for diagnostics and therapeutics. This study characterizes a novel camelid single-domain antibody (nanobody) that binds strongly and with high specificity to rat, mouse and human PSD95. This nanobody allows for a more precise detection and better quantification of PSD95 in various biological samples. We expect that the flexibility and unique performance of this thoroughly characterized affinity tool will help to further understand the role of PSD95 in normal and diseased neuronal synapses.

Competing Interest Statement

Authors of this manuscript are employees or shareholders of NanoTag Biotechnologies GmbH or Synaptic Systems GmbH.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted March 17, 2023.
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Simple and highly efficient detection of PSD95 using a nanobody and its recombinant minibody derivative
Markus Kilisch, Maja Gere-Becker, Liane Wüstefeld, Christel Bonnas, Alexander Crauel, Maja Mechmershausen, Henrik Martens, Hansjörg Götzke, Felipe Opazo, Steffen Frey
bioRxiv 2023.03.16.532938; doi: https://doi.org/10.1101/2023.03.16.532938
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Simple and highly efficient detection of PSD95 using a nanobody and its recombinant minibody derivative
Markus Kilisch, Maja Gere-Becker, Liane Wüstefeld, Christel Bonnas, Alexander Crauel, Maja Mechmershausen, Henrik Martens, Hansjörg Götzke, Felipe Opazo, Steffen Frey
bioRxiv 2023.03.16.532938; doi: https://doi.org/10.1101/2023.03.16.532938

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