Article Information
- March 18, 2023.
Author Information
- Yi Sun1,2,†,
- Michael C. Young1,2,†,
- Claire H. Woodward1,2,†,
- Julia N. Danon1,2,
- Hau Truong3,
- Sagar Gupta1,
- Trenton J. Winters2,
- George Burslem2,3 and
- Nikolaos G. Sgourakis1,2,*
- 1Center for Computational and Genomic Medicine, Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA
- 2Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, 3401 Civic Center Blvd, Philadelphia, PA, 19104, USA
- 3Department of Cancer Biology and Epigenetics Institute, Perelman School of Medicine
- ↵*Correspondence
: Nikolaos G. Sgourakis, Email:
Nikolaos.Sgourakis{at}Pennmedicine.upenn.edu
↵† Contributed equally to this work.
Author Contributions: N.G.S., Y.S., and M.C.Y. designed experiments. Y.S., M.C.Y., and J.N.D. designed plasmids and prepared all recombinant protein samples used in this study. T.J.W. and G.M.B. designed and synthesized peptide probes for FP experiments and refolding reactions. C.H.W. and H.T. performed NMR experiments, resonance assignments, and data analysis. Y.S. performed HDX experiments and data analysis, pMHC-I tetramerization, flow cytometry, and data analysis. J.N.D. performed FP experiments and data analysis. J.N.D. and M.C.Y. performed DSF experiments and data analysis. Y.S., M.C.Y., C.H.W., and N.G.S. wrote the paper, with feedback from all authors. N.G.S. acquired funding and supervised the project.
