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Gel-assisted mass spectrometry imaging

Yat Ho Chan, Koralege C. Pathmasiri, Dominick Pierre-Jacques, View ORCID ProfileStephanie M. Cologna, View ORCID ProfileRuixuan Gao
doi: https://doi.org/10.1101/2023.06.02.543480
Yat Ho Chan
1Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA
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Koralege C. Pathmasiri
1Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA
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Dominick Pierre-Jacques
1Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA
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Stephanie M. Cologna
1Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA
2Laboratory for Integrative Neuroscience, University of Illinois at Chicago; Chicago, IL 60607, USA
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  • ORCID record for Stephanie M. Cologna
Ruixuan Gao
1Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA
2Laboratory for Integrative Neuroscience, University of Illinois at Chicago; Chicago, IL 60607, USA
3Department of Biological Sciences, University of Illinois Chicago; Chicago, IL 60607, USA
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  • ORCID record for Ruixuan Gao
  • For correspondence: gaor@uic.edu
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Abstract

Compatible with label-free detection and quantification, mass spectrometry imaging (MSI) is a powerful tool for spatial investigation of biomolecules in intact specimens. Yet, the spatial resolution of MSI is limited by the method’s physical and instrumental constraints, which often preclude it from single-cell and subcellular applications. By taking advantage of the reversible interaction of analytes with superabsorbent hydrogels, we developed a sample preparation and imaging workflow named Gel-Assisted Mass Spectrometry Imaging (GAMSI) to overcome these limits. With GAMSI, the spatial resolution of lipid and protein MALDI-MSI can be enhanced severalfold without changing the existing mass spectrometry hardware and analysis pipeline. This approach will further enhance the accessibility to (sub)cellular-scale MALDI-MSI-based spatial omics.

Competing Interest Statement

R.G. is a co-inventor on multiple patents related to expansion microscopy. The other authors declare that they have no competing interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 03, 2023.
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Gel-assisted mass spectrometry imaging
Yat Ho Chan, Koralege C. Pathmasiri, Dominick Pierre-Jacques, Stephanie M. Cologna, Ruixuan Gao
bioRxiv 2023.06.02.543480; doi: https://doi.org/10.1101/2023.06.02.543480
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Gel-assisted mass spectrometry imaging
Yat Ho Chan, Koralege C. Pathmasiri, Dominick Pierre-Jacques, Stephanie M. Cologna, Ruixuan Gao
bioRxiv 2023.06.02.543480; doi: https://doi.org/10.1101/2023.06.02.543480

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