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Metagenomic survey of antimicrobial resistance (AMR) in Maryland surface waters differentiated by high and low human impact

Brandon Kocurek, Shawn Behling, Gordon Martin, Padmini Ramachandran, Elizabeth Reed, Christopher Grim, Mark Mammel, Jie Zheng, Alison Franklin, Jay Garland, Daniel A. Tadesse, Manan Sharma, Gregory H. Tyson, Claudine Kabera, Heather Tate, Patrick F. McDermott, Errol Strain, View ORCID ProfileAndrea Ottesen
doi: https://doi.org/10.1101/2023.06.20.545726
Brandon Kocurek
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Shawn Behling
bWashington Department of Fish and Wildlife, Seattle, Washington, USA
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Gordon Martin
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Padmini Ramachandran
cCenter for Food Safety and Applied Nutrition, United states Food and Drug Administration, College Park, Maryland
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Elizabeth Reed
cCenter for Food Safety and Applied Nutrition, United states Food and Drug Administration, College Park, Maryland
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Christopher Grim
cCenter for Food Safety and Applied Nutrition, United states Food and Drug Administration, College Park, Maryland
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Mark Mammel
cCenter for Food Safety and Applied Nutrition, United states Food and Drug Administration, College Park, Maryland
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Jie Zheng
cCenter for Food Safety and Applied Nutrition, United states Food and Drug Administration, College Park, Maryland
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Alison Franklin
dCenter for Environmental Measurement and Modeling, United States Environmental Protection Agency, Cincinnati, Ohio, USA
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Jay Garland
dCenter for Environmental Measurement and Modeling, United States Environmental Protection Agency, Cincinnati, Ohio, USA
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Daniel A. Tadesse
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Manan Sharma
eEnvironmental Microbial & Food Safety Lab, United States Department of Agriculture, Beltsville, MD
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Gregory H. Tyson
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Claudine Kabera
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Heather Tate
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Patrick F. McDermott
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Errol Strain
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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Andrea Ottesen
aCenter for Veterinary Medicine United States, Food and Drug Administration, Laurel, Maryland, USA
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  • ORCID record for Andrea Ottesen
  • For correspondence: Andrea.Ottesen@fda.hhs.gov
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Abstract

In alignment with the One Health paradigm, surface waters are being evaluated as a modality to better understand baseline antimicrobial resistance (AMR) across the environment to supplement existing AMR monitoring in pathogens associated with humans, foods, and animals. Here, we use metagenomic and quasimetagenomic sequence data to describe AMR in Maryland surface waters from developed (high human impact) and natural (low human impact) classifications by the National Land Cover Database (NLCD). Critically important β-lactamase genes were observed in twice as many high human impact zones. All data are available under BioProject PRJNA79347. https://www.ncbi.nlm.nih.gov/bioproject/794347

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, College Park, Maryland, USA

  • The views expressed in this manuscript are those of the authors and do not necessarily reflect the official policy of the Department of Health and Human Services, the U.S. Food and Drug Administration, or the U.S. Government. Reference to any commercial materials, equipment, or process does not in any way constitute approval, endorsement, or recommendation by the Food and Drug Administration.

  • https://www.ncbi.nlm.nih.gov/bioproject/794347

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
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Posted June 20, 2023.
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Metagenomic survey of antimicrobial resistance (AMR) in Maryland surface waters differentiated by high and low human impact
Brandon Kocurek, Shawn Behling, Gordon Martin, Padmini Ramachandran, Elizabeth Reed, Christopher Grim, Mark Mammel, Jie Zheng, Alison Franklin, Jay Garland, Daniel A. Tadesse, Manan Sharma, Gregory H. Tyson, Claudine Kabera, Heather Tate, Patrick F. McDermott, Errol Strain, Andrea Ottesen
bioRxiv 2023.06.20.545726; doi: https://doi.org/10.1101/2023.06.20.545726
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Metagenomic survey of antimicrobial resistance (AMR) in Maryland surface waters differentiated by high and low human impact
Brandon Kocurek, Shawn Behling, Gordon Martin, Padmini Ramachandran, Elizabeth Reed, Christopher Grim, Mark Mammel, Jie Zheng, Alison Franklin, Jay Garland, Daniel A. Tadesse, Manan Sharma, Gregory H. Tyson, Claudine Kabera, Heather Tate, Patrick F. McDermott, Errol Strain, Andrea Ottesen
bioRxiv 2023.06.20.545726; doi: https://doi.org/10.1101/2023.06.20.545726

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