Abstract
ATP-sensitive potassium (KATP) channels, composed of four pore-lining Kir6.2 subunits and four regulatory sulfonylurea receptor 1 (SUR1) subunits, control insulin secretion in pancreatic β-cells. KATP channel opening is stimulated by PIP2 and inhibited by ATP. Mutations that increase channel opening by PIP2 reduce ATP inhibition and cause neonatal diabetes. Although considerable evidence has implicated a role for PIP2 in KATP channel function, previously solved open-channel structures have lacked bound PIP2, and mechanisms by which PIP2 regulates KATP channels remain unresolved. Here, we report cryoEM structure of a KATP channel harboring the neonatal diabetes mutation Kir6.2-Q52R, bound to natural C18:0/C20:4 long-chain PI(4,5)P2 in open conformation. The structure reveals two adjacent PIP2 molecules between SUR1 and Kir6.2. The first PIP2 binding site is conserved among PIP2-gated Kir channels. The second site forms uniquely in KATP at the interface of Kir6.2 and SUR1. Functional studies demonstrate both binding sites determine channel activity. Kir6.2 pore opening is associated with a twist of the Kir6.2 cytoplasmic domain and a rotation of the N-terminal transmembrane domain of SUR1, which widens the inhibitory ATP binding pocket to disfavor ATP binding. The open conformation is particularly stabilized by the Kir6.2-Q52R residue through cation-π bonding with SUR1 - W51. Together, these results uncover the cooperation between SUR1 and Kir6.2 in PIP2 binding and gating, explain the antagonistic regulation of KATP channels by PIP2 and ATP, and provide the mechanism by which Kir6.2-Q52R stabilizes an open channel to cause neonatal diabetes.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Abbreviations: KATP, ATP-sensitive potassium channel; Kir, inward-rectifying potassium channel; SUR1, sulfonylurea receptor 1; CTD, cytoplasmic domain of Kir6.2; KNtp, Kir6.2 N-terminal peptide; TMD, transmembrane domain; NBD, nucleotide binding domain of SUR1; ECL, extracellular loop; ICL, intracellular loop; βA, first part of β-sheet of Kir6.2-CTD; M1 or M2, membrane helix 1 or 2 of Kir6.2; IFH, interfacial helix of Kir6.2; PH, pore helix of Kir6.2; cryoEM, cryo-electron microscopy; TM, transmembrane; PIP2, phosphatidylinositol 4,5-bisphosphate; ATP, adenosine triphosphate.
Figures revised to include additional functional data and structural comparisons; supplemental files updated.