Abstract
The rise of biotechnologies such as gene therapy have brought DNA vectors to the forefront of pharmaceutical development. The quality of the genetic starting material plays a pivotal role in determining the quality of the final product. In this study we examined the fidelity of DNA replication using enzymatic methods (in vitro) compared to plasmid DNA produced in vivo in E. coli. Next-generation sequencing approaches predominantly rely on in vitro polymerases, which have inherent limitations in sensitivity. To address this challenge, we introduce a novel assay based on loss-of-function (LOF) mutations in the conditionally toxic sacB gene. Our findings show that DNA production in E. coli results in significantly fewer LOF mutations (approximately 80-to 3000-fold less) compared to various enzymatic DNA synthesis methods. This includes the most accurate PCR polymerase (Q5) and a commonly employed rolling circle amplification (RCA) DNA polymerase (Phi29). These results suggest that using low-fidelity starting material DNA synthesized in vitro by PCR or RCA may introduce a substantial number of impurities, potentially affecting the quality and yield of final pharmaceutical products. In summary, our study underscores that DNA synthesized in vitro has a significantly higher mutation rate than DNA produced traditionally in E. coli. Therefore, utilizing in vitro enzymatically-produced DNA in biotechnology and biomanufacturing may entail considerable fidelity-related risks, while DNA starting material derived from E. coli substantially mitigates this risk, enhancing overall quality in the production processes.
- DNA Replication Fidelity
- DNA Manufacturing
- DNA Synthesis
- Mutation Rate
- SacB
- E. coli
- Polymerase Chain Reaction (PCR)
- Rolling Circle Amplification (RCA)
- Polymerase
- Loss-of-Function (LOF)
- AAV Manufacturing
- Regulatory Agencies
- Quality
Competing Interest Statement
The authors are employees and own stock in Mediphage Bioceuticals, Inc.