Abstract
Single nucleus RNA-sequencing is critical in deciphering tissue heterogeneity and identifying rare populations. However, current high throughput techniques are not optimized for rare target populations and require tradeoffs in design due to feasibility. We provide a novel snRNA pipeline, MulipleXed Population Selection and Enrichment snRNA-sequencing (XPoSE-seq), to enable targeted snRNA-seq experiments and in-depth transcriptomic characterization of rare target populations while retaining individual sample identity.
Competing Interest Statement
J.C.M., M.K.S., J.W.A, and C.I.C. were employees of BD Biosciences. Manuscript approval by BD Biosciences was not required, and BD Biosciences had no influence regarding data analysis, data interpretation, and discussion. All other authors declare that they do not have any conflicts of interest (financial or otherwise) related to the text of the paper.
