Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: characterization of the enzyme’s in vitro truncation by thiol endopeptidase activity

Brittany S. Menard; Kirsten H. Benidickson; Lee Marie Raytek; Wayne A. Snedden; William C. Plaxton

doi:10.1101/2024.04.11.589046

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Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: characterization of the enzyme’s in vitro truncation by thiol endopeptidase activity

Brittany S. Menard, Kirsten H. Benidickson, Lee Marie Raytek, View ORCID ProfileWayne A. Snedden, View ORCID ProfileWilliam C. Plaxton

doi: https://doi.org/10.1101/2024.04.11.589046

Brittany S. Menard

aDept. of Biology, Queen’s Univ., Kingston, Ontario, Canada, K7L 3N6

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Kirsten H. Benidickson

aDept. of Biology, Queen’s Univ., Kingston, Ontario, Canada, K7L 3N6

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Lee Marie Raytek

bDept. of Plant Sciences, McGill Univ., Ste-Anne-de-Bellevue, Quebec, Canada, H9X 3V9

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Wayne A. Snedden

aDept. of Biology, Queen’s Univ., Kingston, Ontario, Canada, K7L 3N6

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ORCID record for Wayne A. Snedden

William C. Plaxton

aDept. of Biology, Queen’s Univ., Kingston, Ontario, Canada, K7L 3N6

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ORCID record for William C. Plaxton
For correspondence: [email protected]

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Posted April 11, 2024.

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Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: characterization of the enzyme’s in vitro truncation by thiol endopeptidase activity

Brittany S. Menard, Kirsten H. Benidickson, Lee Marie Raytek, Wayne A. Snedden, William C. Plaxton

bioRxiv 2024.04.11.589046; doi: https://doi.org/10.1101/2024.04.11.589046

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Brittany S. Menard, Kirsten H. Benidickson, Lee Marie Raytek, Wayne A. Snedden, William C. Plaxton

bioRxiv 2024.04.11.589046; doi: https://doi.org/10.1101/2024.04.11.589046

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