Abstract
We reanalyzed data from the Slide-tags method developed by Russell et al. and discovered a hidden, spatially informative network formed during the transfer of spatial tags to nuclei. The structure of this network conveys sufficient information to infer cell locations entirely without ground truth from spatial indexing, placing Slide-tags among a new generation of optics-free, network-based imaging-by-sequencing approaches, a fundamental departure from classical spatial sequencing technologies based on pre-indexed arrays.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Posted June 17, 2024.
Hidden network preserved in Slide-tags data allows reference-free spatial reconstruction
