Abstract
The vast majority of gecko species are capable of tail regeneration, but singular geckos of Correlophus, Uroplatus, and Nephrurus genera are unable to regrow lost tails. Of these non-regenerative geckos, the crested gecko (Correlophus ciliatus) is distinguished by ready availability, ease of care, high productivity, and hybridization potential. These features make C. ciliatus particularly suited as a model for studying the genetic, molecular, and cellular mechanisms underlying loss of tail regeneration capabilities. We report a contiguous genome of C. ciliatus with a total size of 1.65 Gb, a total of 152 scaffolds, L50 of 6, and N50 of 109 Mb. Repetitive content consists of 40.41% of the genome, and a total of 30,780 genes were annotated. Assembly of the crested gecko genome provides a valuable resource for future comparative genomic studies between non-regenerative and regenerative geckos and other squamate reptiles.
Findings We report genome sequencing, assembly, and annotation for the crested gecko, Correlophus ciliatus.
Competing Interest Statement
The authors have declared no competing interest.
List of Abbreviations
- BUSCO
- Benchmarking Universal Single-Copy Orthologue
- RNA-Seq
- RNA Sequencing
- BLASTN
- Basic Local Alignment Search Tool (for nucleotides)
- bp
- base pairs
- Mbp
- Mega base pairs
- TE
- transposable element
- hmwDNA
- high molecular weight DNA
- AED
- Annotation Edit Distance