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Comparative analysis of macrophage feeder systems reveals distinct behaviors and key transcriptional shifts in chronic lymphocytic leukemia cells via coculture
Viktoria Kohlhas, Hendrik Jestrabek, View ORCID ProfileRocio Rebollido-Rios, Thanh Tung Truong, Rebekka Zölzer, Luca D. Schreurs, Anton von Lom, View ORCID ProfileAlexander F. vom Stein, Michael Hallek, View ORCID ProfilePhuong-Hien Nguyen
doi: https://doi.org/10.1101/2025.02.13.638101
Viktoria Kohlhas
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Hendrik Jestrabek
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Rocio Rebollido-Rios
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Thanh Tung Truong
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Rebekka Zölzer
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Luca D. Schreurs
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Anton von Lom
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Alexander F. vom Stein
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Michael Hallek
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany
Phuong-Hien Nguyen
1University of Cologne, Faculty of Medicine and University Hospital Cologne, Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf; Center for Molecular Medicine Cologne; CECAD Center of Excellence on Cellular Stress Responses in Aging-Associated Diseases, 50931 Cologne, Germany

Abstract
In this brief report, we evaluated various macrophage coculture systems for their CLL-feeding potential, phagocytosis capacity, induction of treatment resistance in CLL cells, and their impact on the transcriptional profiles of CLL cells.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
↵* Joint first authors
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted February 17, 2025.
Comparative analysis of macrophage feeder systems reveals distinct behaviors and key transcriptional shifts in chronic lymphocytic leukemia cells via coculture
Viktoria Kohlhas, Hendrik Jestrabek, Rocio Rebollido-Rios, Thanh Tung Truong, Rebekka Zölzer, Luca D. Schreurs, Anton von Lom, Alexander F. vom Stein, Michael Hallek, Phuong-Hien Nguyen
bioRxiv 2025.02.13.638101; doi: https://doi.org/10.1101/2025.02.13.638101
Comparative analysis of macrophage feeder systems reveals distinct behaviors and key transcriptional shifts in chronic lymphocytic leukemia cells via coculture
Viktoria Kohlhas, Hendrik Jestrabek, Rocio Rebollido-Rios, Thanh Tung Truong, Rebekka Zölzer, Luca D. Schreurs, Anton von Lom, Alexander F. vom Stein, Michael Hallek, Phuong-Hien Nguyen
bioRxiv 2025.02.13.638101; doi: https://doi.org/10.1101/2025.02.13.638101
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