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The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection

Artemis Perraki, View ORCID ProfileJulien Gronnier, Paul Gouguet, View ORCID ProfileMarie Boudsocq, Anne-Flore Deroubaix, Vincent Simon, Sylvie German-Retana, View ORCID ProfileCyril Zipfel, View ORCID ProfileEmmanuelle Bayer, View ORCID ProfileSébastien Mongrand, Véronique Germain
doi: https://doi.org/10.1101/205765
Artemis Perraki
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
4The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom.
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Julien Gronnier
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Paul Gouguet
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Marie Boudsocq
5Institute of Plant Sciences Paris Saclay (IPS2), CNRS, INRA, Université Paris-Sud, Université d’Evry, Université Paris-Saclay, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, Plateau du Moulon, 91192 Gif sur Yvette, France
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Anne-Flore Deroubaix
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Vincent Simon
3Equipe de Virologie UMR BFP 1332 INRA, 33883 Villenave d’Ornon, France
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Sylvie German-Retana
3Equipe de Virologie UMR BFP 1332 INRA, 33883 Villenave d’Ornon, France
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Cyril Zipfel
4The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom.
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Emmanuelle Bayer
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Sébastien Mongrand
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Véronique Germain
1CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
2Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Bordeaux, France
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Abstract

Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3-s phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 19, 2017.
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The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection
Artemis Perraki, Julien Gronnier, Paul Gouguet, Marie Boudsocq, Anne-Flore Deroubaix, Vincent Simon, Sylvie German-Retana, Cyril Zipfel, Emmanuelle Bayer, Sébastien Mongrand, Véronique Germain
bioRxiv 205765; doi: https://doi.org/10.1101/205765
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The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection
Artemis Perraki, Julien Gronnier, Paul Gouguet, Marie Boudsocq, Anne-Flore Deroubaix, Vincent Simon, Sylvie German-Retana, Cyril Zipfel, Emmanuelle Bayer, Sébastien Mongrand, Véronique Germain
bioRxiv 205765; doi: https://doi.org/10.1101/205765

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