Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate

View ORCID ProfileBenyamin Rosental, Mark Kowarsky, Jun Seita, Daniel M. Corey, Katherine J. Ishizuka, Karla J. Palmeri, Shih-Yu Chen, Rahul Sinha, Jennifer Okamoto, Gary Mantalas, Lucia Manni, Tal Raveh, D. Nathaniel Clarke, Aaron M. Newman, Norma F. Neff, Garry P. Nolan, Stephen R. Quake, Irving L. Weissman, Ayelet Voskoboynik
doi: https://doi.org/10.1101/206318
Benyamin Rosental
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benyamin Rosental
Mark Kowarsky
3Department of Physics, Stanford University, CA 94305, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Seita
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
4AI based Healthcare and Medical Data Analysis Standardization Unit, Medical Sciences Innovation Hub Program, RIKEN, Tokyo 103-0027, Japan.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel M. Corey
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katherine J. Ishizuka
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karla J. Palmeri
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shih-Yu Chen
5Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rahul Sinha
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jennifer Okamoto
6Departments of Applied Physics and Bioengineering, Stanford University, and Chan Zuckerberg Biohub, San Francisco CA 94158, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gary Mantalas
6Departments of Applied Physics and Bioengineering, Stanford University, and Chan Zuckerberg Biohub, San Francisco CA 94158, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lucia Manni
7Dipartimento di Biologia, Università degli Studi di Padova, Padova, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tal Raveh
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. Nathaniel Clarke
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aaron M. Newman
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norma F. Neff
6Departments of Applied Physics and Bioengineering, Stanford University, and Chan Zuckerberg Biohub, San Francisco CA 94158, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Garry P. Nolan
5Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen R. Quake
6Departments of Applied Physics and Bioengineering, Stanford University, and Chan Zuckerberg Biohub, San Francisco CA 94158, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Irving L. Weissman
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ayelet Voskoboynik
1Institute for Stem Cell Biology and Regenerative Medicine, and Ludwig Center, Stanford University School of Medicine.
2Departments of Biology, Stanford University, Hopkins Marine Station, Pacific Grove, CA 93950, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Summary

Hematopoiesis is an essential process that evolved in multicellular animals. At the heart of this process are hematopoietic stem cells (HSCs), which are multipotent, self-renewing and generate the entire repertoire of blood and immune cells throughout life. Here we studied the hematopoietic system of Botryllus schlosseri, a colonial tunicate that has vasculature, circulating blood cells, and interesting characteristics of stem cell biology and immunity. Self-recognition between genetically compatible B. schlosseri colonies leads to the formation of natural parabionts with shared circulation, whereas incompatible colonies reject each other. Using flow-cytometry, whole-transcriptome sequencing of defined cell populations, and diverse functional assays, we identified HSCs, progenitors, immune-effector cells, the HSC niche, and demonstrated that self-recognition inhibits cytotoxic reaction. Our study implies that the HSC and myeloid lineages emerged in a common ancestor of tunicates and vertebrates and suggests that hematopoietic bone marrow and the B. schlosseri endostyle niche evolved from the same origin.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 26, 2017.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate
Benyamin Rosental, Mark Kowarsky, Jun Seita, Daniel M. Corey, Katherine J. Ishizuka, Karla J. Palmeri, Shih-Yu Chen, Rahul Sinha, Jennifer Okamoto, Gary Mantalas, Lucia Manni, Tal Raveh, D. Nathaniel Clarke, Aaron M. Newman, Norma F. Neff, Garry P. Nolan, Stephen R. Quake, Irving L. Weissman, Ayelet Voskoboynik
bioRxiv 206318; doi: https://doi.org/10.1101/206318
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate
Benyamin Rosental, Mark Kowarsky, Jun Seita, Daniel M. Corey, Katherine J. Ishizuka, Karla J. Palmeri, Shih-Yu Chen, Rahul Sinha, Jennifer Okamoto, Gary Mantalas, Lucia Manni, Tal Raveh, D. Nathaniel Clarke, Aaron M. Newman, Norma F. Neff, Garry P. Nolan, Stephen R. Quake, Irving L. Weissman, Ayelet Voskoboynik
bioRxiv 206318; doi: https://doi.org/10.1101/206318

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4667)
  • Biochemistry (10332)
  • Bioengineering (7650)
  • Bioinformatics (26277)
  • Biophysics (13495)
  • Cancer Biology (10663)
  • Cell Biology (15388)
  • Clinical Trials (138)
  • Developmental Biology (8477)
  • Ecology (12797)
  • Epidemiology (2067)
  • Evolutionary Biology (16816)
  • Genetics (11378)
  • Genomics (15450)
  • Immunology (10591)
  • Microbiology (25139)
  • Molecular Biology (10186)
  • Neuroscience (54314)
  • Paleontology (399)
  • Pathology (1663)
  • Pharmacology and Toxicology (2889)
  • Physiology (4331)
  • Plant Biology (9223)
  • Scientific Communication and Education (1585)
  • Synthetic Biology (2551)
  • Systems Biology (6768)
  • Zoology (1459)