Systematic Dissection of Sequence Elements Controlling σ70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli

Guillaume Urtecho; Arielle D. Tripp; Kimberly D. Insigne; Hwangbeom Kim; Sriram Kosuri

doi:10.1101/207332

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Systematic Dissection of Sequence Elements Controlling σ70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli

Guillaume Urtecho, Arielle D. Tripp, Kimberly D. Insigne, Hwangbeom Kim, View ORCID ProfileSriram Kosuri

doi: https://doi.org/10.1101/207332

Guillaume Urtecho

1Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, CA, 90095, USAvvedens

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Arielle D. Tripp

2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA, 90095, USA

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Kimberly D. Insigne

3Bioinformatics Interdepartmental Graduate Program, University of California, Los Angeles, CA 90095, USA

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Hwangbeom Kim

4Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA

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Sriram Kosuri

4Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA

5UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, Quantitative and Computational Biology Institute, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA