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Characterization of a Novel Model of Overuse-Induced Calcific Achilles Tendinopathy in Mice: Contralateral Tendinopathy Following Unilateral Tenotomy

Liang Wang, Jie Zhang, Gang-hui Yin, Zhong-min Zhang, Tian-yu Chen, Jian Jin, Ping-lin Lai, Bin Huang, Bo Yan, Yu-hui Chen, Da-di Jin, View ORCID ProfileMin-jun Huang
doi: https://doi.org/10.1101/209247
Liang Wang
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Jie Zhang
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Gang-hui Yin
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Zhong-min Zhang
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Tian-yu Chen
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Jian Jin
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
dDepartment of Spine Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, PR China
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Ping-lin Lai
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
cDepartment of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, Guangdong, PR China
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Bin Huang
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Bo Yan
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Yu-hui Chen
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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Da-di Jin
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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  • For correspondence: jindadi@yahoo.cn hmjlceb@163.com
Min-jun Huang
aDepartment of Orthopedics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510665 Guangdong, PR China
bAcademy of Orthopedics, Guangdong Province, Guangzhou 510665, Guangdong, PR USA
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  • ORCID record for Min-jun Huang
  • For correspondence: jindadi@yahoo.cn hmjlceb@163.com
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Abstract

Objective To develop a simple but reproducible overuse induced animal model of Achilles tendinopathy in mice for better understanding the underlying mechanism and prevention of calcific Achilles tendinopathy.

Methods 80 C57/B6 mice (8-9 weeks old) were employed and randomly divided into control group and experimental group. Unilateral Achilles tenotomy was performed on the right hindlimb of experiment group. After 12 weeks, the onset of Achilles tedinopathy in the contralateral Achilles tendon was determined by radiological assessment, histological analysis, electron microscopy observation and biomechanical test.

Results The onset of calcific Achilles tendinopathy in contralateral Achilles tendon was confirmed after 12 weeks unilateral tenotomy. The contralateral Achilles tendon of experimental group was characterized as hypercelluarity, neovascularization and fused collagen fiber disarrangement, compared to the control group. Importantly, intratendon endochondral ossification and calcaneus deformity was featured in contralateral Achilles tendon. Additionally, poor biomechanical properties in the contralateral Achilles tendon revealed the incidence of Achilles tedinopathy.

Conclusion We hereby introduce a novel simple but reproducible spontaneous contralateral calcific Achilles tendinopathy model in mice, which represents the overuse conditions during the tendinopathy development in human-beings. It should be a useful tool to further study the underlying pathogenesis of calcific Achilles tendinopathy.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 26, 2017.
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Characterization of a Novel Model of Overuse-Induced Calcific Achilles Tendinopathy in Mice: Contralateral Tendinopathy Following Unilateral Tenotomy
Liang Wang, Jie Zhang, Gang-hui Yin, Zhong-min Zhang, Tian-yu Chen, Jian Jin, Ping-lin Lai, Bin Huang, Bo Yan, Yu-hui Chen, Da-di Jin, Min-jun Huang
bioRxiv 209247; doi: https://doi.org/10.1101/209247
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Characterization of a Novel Model of Overuse-Induced Calcific Achilles Tendinopathy in Mice: Contralateral Tendinopathy Following Unilateral Tenotomy
Liang Wang, Jie Zhang, Gang-hui Yin, Zhong-min Zhang, Tian-yu Chen, Jian Jin, Ping-lin Lai, Bin Huang, Bo Yan, Yu-hui Chen, Da-di Jin, Min-jun Huang
bioRxiv 209247; doi: https://doi.org/10.1101/209247

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