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CHRAC/ACF Contribute to the Repressive Ground State of Chromatin

Alessandro Scacchetti, Laura Brueckner, Dhawal Jain, Tamas Schauer, Xu Zhang, Frank Schnorrer, Bas van Steensel, Tobias Straub, Peter B. Becker
doi: https://doi.org/10.1101/218768
Alessandro Scacchetti
Molecular Biology Div., Biomedical Ctr., Faculty of Medicine, LMU Munich, Germany;
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Laura Brueckner
Div. of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands;
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Dhawal Jain
Molecular Biology Div., Biomedical Ctr., Faculty of Medicine, LMU Munich, Germany;
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Tamas Schauer
Molecular Biology Div., Biomedical Ctr., Faculty of Medicine, LMU Munich, Germany;
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Xu Zhang
School of Life Science and Engineering, Foshan University, China;
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Frank Schnorrer
Developmental Biology Institute of Marseille, Aix Marseille Univ, CNRS, France;
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Bas van Steensel
Div. of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands;
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Tobias Straub
Bioinformatic Unit, Biomedical Center, Faculty of Medicine, LMU Munich, Germany
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Peter B. Becker
Molecular Biology Div., Biomedical Ctr., Faculty of Medicine, LMU Munich, Germany;
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  • For correspondence: pbecker@bmc.med.lmu.de
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Abstract

The chromatin remodeling complexes CHRAC and ACF combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression is unclear. Here we explored the influence of Drosophila CHRAC/ACF on transcription by complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcriptome analysis of a Acf knock-out allele showed that only lowly expressed genes are de-repressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.

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Posted November 13, 2017.
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CHRAC/ACF Contribute to the Repressive Ground State of Chromatin
Alessandro Scacchetti, Laura Brueckner, Dhawal Jain, Tamas Schauer, Xu Zhang, Frank Schnorrer, Bas van Steensel, Tobias Straub, Peter B. Becker
bioRxiv 218768; doi: https://doi.org/10.1101/218768
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CHRAC/ACF Contribute to the Repressive Ground State of Chromatin
Alessandro Scacchetti, Laura Brueckner, Dhawal Jain, Tamas Schauer, Xu Zhang, Frank Schnorrer, Bas van Steensel, Tobias Straub, Peter B. Becker
bioRxiv 218768; doi: https://doi.org/10.1101/218768

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