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Genetic Single Neuron Anatomy reveals fine granularity of cortical interneuron subtypes

Xiaojun Wang, Jason Tucciarone, Siqi Jiang, Fangfang Yin, Bor-shuen Wang, Dingkang Wang, Yao Jia, Xueyan Jia, Yuxin Li, Tao Yang, Zhengchao Xu, Masood A. Akram, Yusu Wang, Shaoqun Zeng, Giorgio A. Ascoli, Partha Mitra, Hui Gong, Qingming Luo, Z. Josh Huang
doi: https://doi.org/10.1101/219485
Xiaojun Wang
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Jason Tucciarone
3Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
4Program in Neuroscience and Medical Scientist Training Program, Stony Brook University, New York 11790, USA
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Siqi Jiang
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Fangfang Yin
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Bor-shuen Wang
3Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
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Dingkang Wang
5Computer Science and Engineering Department, The Ohio State University, Columbus, OH 43221, United States of America.
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Yao Jia
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Xueyan Jia
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Yuxin Li
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Tao Yang
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Zhengchao Xu
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Masood A. Akram
6Center for Neural Informatics, Structures & Plasticity, Krasnow Institute for Advanced Study, George Mason University, Fairfax, VA (USA).
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Yusu Wang
5Computer Science and Engineering Department, The Ohio State University, Columbus, OH 43221, United States of America.
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Shaoqun Zeng
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Giorgio A. Ascoli
6Center for Neural Informatics, Structures & Plasticity, Krasnow Institute for Advanced Study, George Mason University, Fairfax, VA (USA).
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Partha Mitra
3Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
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Hui Gong
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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Qingming Luo
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
2Department of Biomedical Engineering, Key Laboratory for Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
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  • For correspondence: huangj@cshl.edu qluo@mail.hust.edu.cn
Z. Josh Huang
3Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
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  • For correspondence: huangj@cshl.edu qluo@mail.hust.edu.cn
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Abstract

Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of discrete cell groups from population continuum. Combining genetic labeling with high-resolution, large volume light microscopy, we established a platform of genetic single neuron anatomy that resolves, registers and quantifies complete neuron morphologies in the mouse brain. We discovered that cortical axo-axonic cells (AACs), a cardinal GABAergic interneuron type that controls pyramidal neuron (PyN) spiking at axon initial segment, consist of multiple subtypes distinguished by laminar position, dendritic and axonal arborization patterns. Whereas the laminar arrangements of AAC dendrites reflect differential recruitment by input streams, the laminar distribution and local geometry of AAC axons enable differential innervation of PyN ensembles. Therefore, interneuron types likely consist of fine-grained subtypes with distinct input-output connectivity patterns.

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Posted November 20, 2017.
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Genetic Single Neuron Anatomy reveals fine granularity of cortical interneuron subtypes
Xiaojun Wang, Jason Tucciarone, Siqi Jiang, Fangfang Yin, Bor-shuen Wang, Dingkang Wang, Yao Jia, Xueyan Jia, Yuxin Li, Tao Yang, Zhengchao Xu, Masood A. Akram, Yusu Wang, Shaoqun Zeng, Giorgio A. Ascoli, Partha Mitra, Hui Gong, Qingming Luo, Z. Josh Huang
bioRxiv 219485; doi: https://doi.org/10.1101/219485
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Genetic Single Neuron Anatomy reveals fine granularity of cortical interneuron subtypes
Xiaojun Wang, Jason Tucciarone, Siqi Jiang, Fangfang Yin, Bor-shuen Wang, Dingkang Wang, Yao Jia, Xueyan Jia, Yuxin Li, Tao Yang, Zhengchao Xu, Masood A. Akram, Yusu Wang, Shaoqun Zeng, Giorgio A. Ascoli, Partha Mitra, Hui Gong, Qingming Luo, Z. Josh Huang
bioRxiv 219485; doi: https://doi.org/10.1101/219485

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